sulfo-Cyanine3 tyramide
| 貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
|---|---|---|---|---|
| 3718-1mg | 1 mg | $110 | 現貨 |
Tyramide signal amplification (TSA) is the most versatile and effective way to enhance the intensity of the fluorescent signal, used in immunohistochemistry (IHC), immunocytochemistry (ICC), and fluorescence in situ hybridization (FISH). The TSA method is based on the ability of horseradish peroxidase (HRP) in the presence of low concentrations of hydrogen peroxide to convert a labeled tyramine-containing substrate into an oxidized, highly reactive free radical that covalently binds to the tyrosine residues of protein molecules located next to it.
Compared to conventional procedures, the TSA method increases the sensitivity of immunofluorescent detection of target molecules by more than 100 times, making it particularly suitable for detecting low-concentration targets. In applications where increased detection sensitivity is not required, TSA can significantly reduce antibody or probe concentrations without loss of signal intensity, thereby reducing background staining due to cross-reactivity or non-specific binding of antibodies.
Since the binding of the tyramide label is covalent, tyramides of different dyes can be used in several sequential rounds of TSA staining to detect multiple targets in the same sample.
This tyramide is a conjugate of the water-soluble orange fluorescent dye sulfo-Cyanine3. sulfo-Cyanine3 tyramide (also known as Cy3® and Cyanine3 tyramide from other manufacturers) is a component of many tyramide signal amplification (TSA) kits. It can be used with any antibody or other molecules (streptavidin, etc.) conjugated to HRP to stain cells and tissues by immunofluorescence methods.
顧客還購買了這個產品
AF 488 馬來醯亞胺 (AF 488 maleimide)
AF 488 C6 馬來醯亞胺是一種用於標記蛋白質 SH 基團的硫醇反應性活性染料。Cyanine3疊氮化物 (Cyanine3 azide)
用於 сlick сhemistry 的 Cyanine3 螢光染料的疊氮衍生物。Linear Polyacrylamide for Nucleic Acid Precipitation
Nucleic acid coprecipitant. Suitable for PCR and RT-PCR reactions.General properties
| Appearance: | red powder |
| Molecular weight: | 774.02 |
| Molecular formula: | C38H44KN3O8S2 |
| Solubility: | soluble in water, DMSO, DMF |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
| MSDS: | 下載 |
| 產品規格 |
Spectral properties
| Excitation/absorption maximum, nm: | 548 |
| ε, L⋅mol−1⋅cm−1: | 162000 |
| Emission maximum, nm: | 563 |
| Fluorescence quantum yield: | 0.1 |
| CF260: | 0.03 |
| CF280: | 0.06 |
您的購物車是空的
$ 
