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sulfo-Cyanine5 hydrazide

醯肼類磺基-花菁5

貨號	數量	價格	交貨時間
13370	1 mg	$125	現貨
23370	5 mg	$325	現貨
43370	25 mg	$850	現貨
53370	50 mg	$1490	現貨
63370	100 mg	$1990	現貨

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sulfo-Cyanine5 is a sulfated derivative of Cyanine5 dye, well soluble in water because of two negatively charged sulfo groups in its structure. By its spectral characteristics, this far-red fluorescent dye is an analog of Cy™5.

Hydrazides efficiently react with aldehydes and ketones resulting in hydrazones, so this compound can be used for conjugation with carbonyl derivatives of biomolecules.

The reaction runs in aqueous conditions, which is important when working with antibodies and proteins. Cys-diol groups in sugars in glycosylated proteins and antibodies can be oxidized into dialdehydes, and cysteine in proteins can be converted with enzymes to formyl glycerol (i. e. reactive groups for conjugation with sulfo-Cyanine5 hydrazide). Carboxyl groups of aspartic and glutamic acids in proteins and peptides can be also conjugated with sulfo-Cyanine5 hydrazide in the presence of activating agents: carbodiimide (EDAC) or methyl morpholine (DMTMM) derivatives.

以
ChemDraw 格式下載

General properties

Appearance:	dark blue solid
Molecular weight:	656.83
CAS number:	2055138-61-7
Molecular formula:	C₃₂H₄₀N₄O₇S₂
Solubility:	very good in water, good in DMF and DMSO
Quality control:	NMR ¹H and HPLC-MS (95+%)
Storage conditions:	24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
MSDS:	下載
	產品規格

Spectral properties

Excitation/absorption maximum, nm:	646
ε, L⋅mol⁻¹⋅cm⁻¹:	271000
Emission maximum, nm:	662
Fluorescence quantum yield:	0.28
CF₂₆₀:	0.04
CF₂₈₀:	0.04