Di-4-ANEPPS, potentiometric probe
貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
---|---|---|---|---|
3429-50ug | 50 ug | – | 現貨 | |
3429-5mg | 5 mg | $225 | 現貨 |
Di-4-ANEPPS is an Amino-Naphthyl-Ethenyl-Pyridinium (ANEP) family voltage-sensitive dye widely used as a fast-responding membrane potential probe. The dye is non-fluorescent until bound to membranes and fluoresces only in response to electrical potential fluctuations in its environment.
The optical response of Di-4-ANEPPS is fast enough to detect transient (millisecond) potential changes in excitable cells, such as single neurons, cardiac cells, and intact brains. The magnitude of potential-dependent fluorescence change is about 2-10% per 100 mV. The dye also displays a potential-dependent shift in excitation spectrum, permitting the quantitation of cell membrane potential using ratiometric techniques.
Di-4-ANEPPS is quickly internalized by cells, so it is primarily used for short-term studies. We also provide Di-8-ANEPPS, which is more hydrophobic and better retained in the outer leaflet of the cell membrane. Since Di-4-ANEPPS binds to the cell membrane, it can also be used as a plasma membrane and endocytosis marker.
Excitation/emission maxima of Di-4-ANEPPS in methanol are 496/705 nm, respectively. In lipids and cell membranes, the excitation and emission spectra of the dye are typically blue-shifted compared to organic solvent.
Di-4-ANEPPS can be introduced into cells by directly adding the stock solution to the culture medium, using Pluronic® F-127, or retrograde labeling. Use a 5-10 μM working concentration as a starting point. The exact dye concentration should be defined experimentally.
顧客還購買了這個產品
RH 237,電位探針 (RH 237, potentiometric probe)
快速響應的電位敏感螢光探針,用於對線粒體、神經元和心臟細胞的功能活動進行成像。Di-8-ANEPPS, potentiometric probe
Fast-response potential-sensitive fluorescent probe for imaging functional activity of neurons and heart cells. The dye has more lipophilic properties than other dyes of the ANEP family, making it well-suited for long-term experiments.General properties
Appearance: | red solid |
Molecular weight: | 480.67 |
CAS number: | 90134-00-2 |
Molecular formula: | C28H36N2O3S |
Solubility: | ethanol, DMF, DMSO |
Quality control: | NMR 1H and HPLC-MS (95+%) |
Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
MSDS: | 下載 |
產品規格 |