DDTT Sulfurizing Reagent
貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
---|---|---|---|---|
3179-1g | 1 g | – | 現貨 | |
3179-5g | 5 g | $245 | 現貨 | |
3179-25g | 25 g |
$1150
|
現貨 | |
3179-50g | 50 g |
$1800
|
現貨 | |
3179-100g | 100 g | 請查詢 | 現貨 |
DDTT is a sulfurizing reagent used to prepare phosphorothioate linkages, which increase the stability and nuclease resistance of nucleic acids, especially of RNA. It is suitable for automated synthesizers employing standard phosphoramidites for a large-scale synthesis of oligonucleotides.
DDTT demonstrates faster kinetics and higher stability in solution, compared to other sulfurizing reagents such as tetraethylthiuram disulfide (TETD) and Beaucage reagent, respectively. DDTT is commonly applied as a 0.05 M or 0.1 M solution in pyridine/acetonitrile and has a lifetime of up to 6 months.
Usage
Coupling: Sulfurization step must come before capping step. The optimum sulfurizing time for DDTT Sulfurizing Reagent, 0.05 M solution in pyridine/acetonitrile: 1 min for DNA or 6 min for RNA.
Deprotection: standard conditions.
顧客還購買了這個產品
JOE NHS ester, 5-isomer
JOE is a fluorescent dye with emission in the yellow part of the spectrum. This derivative is an activated NHS ester for conjugation with amines.DusQ® 2 亞磷醯胺 (DusQ® 2 phosphoramidite)
DusQ® 2 亞磷醯胺用於合成在 3' 端、5' 端和內部位置用 DusQ® 2 淬滅劑標記的寡核苷酸。該亞磷醯胺具有DMT保護,可用於小柱純化。立即在線獲取免費快遞服務
通用 CPG II 型,1000A (Universal CPG type II, 1000A)
這種通用支持物旨在用於寡核苷酸的自動合成,以加速解封閉過程中的去磷酸化過程。對於多達 120 個堿基的寡核苷酸合成,建議使用 1000 Å 的孔徑。General properties
Appearance: | lemon yellow crystals |
Molecular weight: | 205.33 |
CAS number: | 1192027-04-5 |
Molecular formula: | C5H7N3S3 |
Quality control: | NMR 1H and HPLC-MS (95+%) |
Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
MSDS: | 下載 |
產品規格 |
Oligo synthesis details
Diluent: | acetonitrile |
Coupling conditions: | standard coupling, identical to normal nucleobases |
Deprotection conditions: | identical to protected nucleobases |
