DDTT Sulfurizing Reagent
| 貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
|---|---|---|---|---|
| 3179-1g | 1 g | – | 現貨 | |
| 3179-5g | 5 g | $245 | 現貨 | |
| 3179-25g | 25 g |
$1150
|
現貨 | |
| 3179-50g | 50 g |
$1800
|
現貨 | |
| 3179-100g | 100 g | 請查詢 | 現貨 |
DDTT is a sulfurizing reagent used to prepare phosphorothioate linkages, which increase the stability and nuclease resistance of nucleic acids, especially of RNA. It is suitable for automated synthesizers employing standard phosphoramidites for a large-scale synthesis of oligonucleotides.
DDTT demonstrates faster kinetics and higher stability in solution, compared to other sulfurizing reagents such as tetraethylthiuram disulfide (TETD) and Beaucage reagent, respectively. DDTT is commonly applied as a 0.05 M or 0.1 M solution in pyridine/acetonitrile and has a lifetime of up to 6 months.
Usage
Coupling: Sulfurization step must come before capping step. The optimum sulfurizing time for DDTT Sulfurizing Reagent, 0.05 M solution in pyridine/acetonitrile: 1 min for DNA or 6 min for RNA.
Deprotection: standard conditions.
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通用 CPG II 型，1000A (Universal CPG type II, 1000A)
這種通用支持物旨在用於寡核苷酸的自動合成，以加速解封閉過程中的去磷酸化過程。對於多達 120 個堿基的寡核苷酸合成，建議使用 1000 Å 的孔徑。General properties
| Appearance: | lemon yellow crystals |
| Molecular weight: | 205.33 |
| CAS number: | 1192027-04-5 |
| Molecular formula: | C5H7N3S3 |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | 下載 |
| 產品規格 |
Oligo synthesis details
| Diluent: | acetonitrile |
| Coupling conditions: | standard coupling, identical to normal nucleobases |
| Deprotection conditions: | identical to protected nucleobases |
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