DDTT Sulfurizing Reagent
| 貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
|---|---|---|---|---|
| 3179-1g | 1 g |
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|
現貨 | |
| 3179-5g | 5 g |
$245
|
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| 3179-25g | 25 g |
$1150
|
現貨 | |
| 3179-50g | 50 g |
$1800
|
現貨 | |
| 3179-100g | 100 g |
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現貨 |
DDTT is a sulfurizing reagent used to prepare phosphorothioate linkages, which increase the stability and nuclease resistance of nucleic acids, especially of RNA. It is suitable for automated synthesizers employing standard phosphoramidites for a large-scale synthesis of oligonucleotides.
DDTT demonstrates faster kinetics and higher stability in solution, compared to other sulfurizing reagents such as tetraethylthiuram disulfide (TETD) and Beaucage reagent, respectively. DDTT is commonly applied as a 0.05 M or 0.1 M solution in pyridine/acetonitrile and has a lifetime of up to 6 months.
Usage
Coupling: Sulfurization step must come before capping step. The optimum sulfurizing time for DDTT Sulfurizing Reagent, 0.05 M solution in pyridine/acetonitrile: 1 min for DNA or 6 min for RNA.
Deprotection: standard conditions.
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Universal CPG type I, 500A
This universal support is intended for the automated synthesis of all types of oligonucleotides. Pore size of 500 Å is recommended for the synthesis of oligonucleotides up to 50 bases in length.磺基-Cyanine7 NHS 酯 (sulfo-Cyanine7 NHS ester)
sulfo-Cyanine7 NHS 酯是一種水溶性、近紅外、胺反應性染料。Phosphate CPG 1000 solid support
Phosphate modifier CPG for the solid phase synthesis of long (up to 120mer) oligonucleotides containing 3 prime phosphate modification.General properties
| Appearance: | lemon yellow crystals |
| Molecular weight: | 205.33 |
| CAS number: | 1192027-04-5 |
| Molecular formula: | C5H7N3S3 |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | 下載 |
| 產品規格 |
Oligo synthesis details
| Diluent: | acetonitrile |
| Coupling conditions: | standard coupling, identical to normal nucleobases |
| Deprotection conditions: | identical to protected nucleobases |
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