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• Biotinylating Everything: Lumiprobe's biotinylation product line

Biotinylating Everything: Lumiprobe's biotinylation product line

Biotinylation is a key technology in modern biology, allowing the specific labeling of proteins, antibodies, nucleic acids, and other molecules with biotin. This label binds with high affinity and specificity to avidin, streptavidin, or neutravidin, forming the basis for numerous applications:

• Immunodetection: Signal amplification in ELISA, Western blot, immunohistochemistry (IHC), and flow cytometry (FACS).
• Affinity Purification: Isolation of specific targets (e.g., biotinylated DNA/RNA, proteins) from complex mixtures using avidin-containing resins.
• Immobilization: Fixation of biotinylated molecules onto (strept)avidin-coated surfaces for biosensors or microarrays.
• Interaction Studies: Investigation of protein-protein or protein-nucleic acid interactions.

We have developed a line of biotinylation products, including:

Biotin NHS esters with different spacer lengths for flexible labeling amino groups:

Biotins for labeling sulfhydryl and carbonyl groups:

Biotins for highly specific labeling via azide-alkyne cycloaddition reaction (click chemistry):

Biotinylated components for oligonucleotide synthesis:

As well as streptavidins conjugated with fluorophores for detection of biotinylated targets:

Advantages of Lumiprobe Biotinylation Reagents:

• High Purity & Stability: Guaranteed quality for reproducible results.
• Broad Selection: Solutions for virtually any labeling strategy and target molecule type.
• Optimized Spacers: PEG spacers minimize steric hindrance, enhancing biotin tag accessibility for (strept)avidin binding.
• Competitive Pricing: Cost-effective solutions without compromising quality.

For detailed information on biotinylation products and ordering, visit our catalog.

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