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EdU Cell Proliferation Kit

Methods for quantifying cell proliferation are widely used in cytotoxicity studies, oncology research, and other areas of cell biology. Many of them allow the visualization of proliferating cells using fluorescent dyes and can be used for high-throughput screening.

Detection of DNA replicated during the S-phase of the cell cycle is one of the most direct ways to detect cell proliferation. Typically, 5-bromo-2’-deoxyuridine (BrdU), a synthetic analog of thymidine that is incorporated into the daughter strands of DNA during DNA replication, is used for this purpose. BrdU-containing DNA is then detected immunochemically using antibodies specific to this nucleoside.

A quick and simple alternative to immunochemistry is to label dividing cells with another nucleoside, 5-ethynyl-2’-deoxyuridine (EdU). Like BrdU, it can be delivered into cells by adding it to culture media or injecting it into animals. EdU is also a substrate of cellular kinases and DNA polymerases, due to which it is incorporated into DNA during its replication. After this, the DNA of the daughter cells containing the ethynyl fragments can be labeled using a click reaction with fluorescent azides in the presence of a copper catalyst.

Cells labeled in this way can be detected by microscopy and cytometry or used to sort daughter cells and cells that have not undergone division.

Lumiprobe offers a ready-to-use EdU Cell Proliferation Kit in five variants with different fluorescent dyes.

Each kit contains:

• 5-Ethynyl-2’-deoxyuridine (EdU), an analog of thymidine;
• Cu(II)-BTTAA complex, a catalyst for click reaction;
• Ascorbic acid, a reducing agent for copper;
• An azide of one of the fluorescent dyes — AF 488 (Cat. 21272), AF 555 (Cat. 22272), AF 594 (Cat. 23272), or AF 647 (Cat. 24272), or a set of four azides — the All-Colors kit (Cat. .2S272);
• DMSO, dimethyl sulfoxide labeling grade, a solvent for azides;
• DAPI, blue fluorescent nuclear dye.