CFDA SE Cell Tracing Kit
| 貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
|---|---|---|---|---|
| 16231 |
3 vials
|
– | 1 天 | |
| 26231 |
15 vials
|
$230 | 1 天 |
CFDA SE Cell Tracing Kit is used for fluorescent labeling and long-term tracing of cells.
CFDA SE ((5,6)-carboxyfluorescein diacetate succinimidyl ester) is a stable, cell-permeable diacetate precursor to CFSE. This molecule is non-fluorescent until the acetate groups are cleaved by intracellular esterases resulting in the formation of a highly fluorescent fluorophore CFSE with emission in the green spectrum range (absorption max. at ~492 nm, emission max. at ~517 nm). CFSE interacts with cellular amines via its succinimidyl groups and covalently labels intracellular proteins.
CFDA SE is commonly used for in vivo and in vitro labeling of cells to analyze their proliferation as well as for cell tracking and motility assays. Inside the cell, CFDA SE shows little cytotoxicity, with minimal effects on the proliferative ability or biology of the cell. During proliferation, the label is partitioned approximately equally among the progeny so that cell division can be followed as a successive halving of the fluorescence intensity through multiple generational divisions.
CFDA SE cell tracing kit contains single-use vials of dry dye and the required amount of high-quality anhydrous DMSO. It allows the preparation of small volumes of the working staining solution, which is convenient for conducting experiments and scaling them up without unnecessary loss of the dye.
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The ready-to-use kit to label cell membranes with red fluorescent PKH26 dye for evaluating cell migration or proliferation.CytoTracer Green CM-BDP®
Non-toxic, cell-permeant green fluorescent dye for labeling and long-term tracing of living cells.| 储存条件: | |
| MSDS: | 下載 |
| 激发/吸收极大值，纳米: | 492 |
| 发射极大值，纳米: | 517 |
| 储存时间（月: | 12 |
參考資料
- Leshchenko, E.V.; Chingizova, E.A.; Antonov, A.S.; Shlyk, N.P.; Borkunov, G.V.; Berdyshev, D.V.; Chausova, V.E.; Kirichuk, N.N.; Khudyakova, Y.V.; Chingizov, A.R.; Kalinovsky, A.I.; Popov, R.S.; Kim, N.Y..; Chadova, K.A.; Yurchenko, E.A.; Isaeva, M.P.; Yurchenko, A.N. New Zosteropenillines and Pallidopenillines from the Seagrass-Derived Fungus Penicillium Yezoense KMM 4679. Marine Drugs, 2024, 22(7), 317. doi: 10.3390/md22070317
- Chingizova, E. A.; Yurchenko, E. A.; Chingizov, A. R.; Klimovich, A. A.; Pislyagin, E. A.; Menchinskaya, E. S.; Kuzmich, A. S.; Trinh, P. T. H.; Ngoc, N. T. D.; Van, T. T. T.; Guzhova, I. V.; Aminin, D. L.; Yurchenko, A. N. The Effects of Marine Fungal Asterripeptides A–C on In Vitro and In Vivo Staphylococcus Aureus Skin Infection. Pharmaceuticals, 2024, 17(10), 1345. doi: 10.3390/ph17101345
- Chingizova, E. A.; Chingizov, A. R.; Menchinskaya, E. S.; Pislyagin, E. A.; Kuzmich, A. S.; Leshchenko, E. V.; Borkunov, G. V.; Guzhova, I. V.; Aminin, D. L.; Yurchenko, E. A. The Influence of Marine Fungal Meroterpenoid Meroantarctine A toward HaCaT Keratinocytes Infected with Staphylococcus Aureus. J Antibiot, 2024, 77(12), 812–822. doi: 10.1038/s41429-024-00771-x
- Girich, E.V.; Trinh, P.T.H; Nesterenko, L.E.; Popov, R.S.; Kim, N.Y.; Rasin, A.B.; Menchinskaya, E.S.; Kuzmich, A.S.; Chingizova, E.A.; Minin, A.S.; Ngoc, N.T.D.; Van, T.T.T.; Yurchenko, E.A.; Yurchenko, A.N.; Berdyshev, D.V. Absolute Stereochemistry and Cytotoxic Effects of Vismione E from Marine Sponge-Derived Fungus Aspergillus sp. 1901NT-1.2.2. International Journal of Molecular Sciences, 2023, 24(9), 8150. doi: 10.3390/ijms24098150
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