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WST-8, reagent for cell proliferation assay

Cell viability and apoptosis Cell proliferation

貨號	數量	價格	交貨時間	購買此產品
4868-100mg	100 mg	$105	現貨
4868-500mg	500 mg	$980	現貨

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WST-8 (Water-Soluble Tetrazolium 8) is a water-soluble tetrazolium salt widely used to assess the metabolic activity of cells. The dye does not penetrate living cells but can be reduced outside the cells by NADPH-dependent cellular oxidoreductases to water-soluble formazan. The reaction occurs by electron transfer across the plasma membrane in a neutral pH and the presence of an intermediate electron acceptor, 1-methoxyphenazine methosulfate. The staining intensity is proportional to the number of viable cells. The maximum absorption of the reaction product is 450–500 nm.

The use of WST-8 has several advantages:

Unlike MTT, working with WST-8 does not require dissolving formazan crystals, simplifying the protocol, and eliminating the use of toxic solvents (e.g., DMSO).
Cell analysis using WST-8 is a one-step method. The reagent is added directly to the culture medium, and the results are read without additional processing.
WST-8 is non-toxic to cells, which allows for long incubations (up to 24 hours) without the risk of artifacts.
High sensitivity of the method. A wide dynamic range ensures accuracy even at low cell density.
The method is compatible with adherent and suspension cultures and various types of spectrophotometric measurement plates.

We also supply WST-8 as a ready-to-use kit for cell proliferation assay.

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CytoTracer 綠色 CM-BDP® (CytoTracer Green CM-BDP®)

無毒、細胞滲透性綠色螢光染料，用於活細胞的標記和長期示蹤。

General properties

Appearance:	brick solid
Molecular weight:	600.47
CAS number:	193149-74-5
Molecular formula:	C₂₀H₁₄N₆NaO₁₁S₂
IUPAC name:	2-(2-Methoxy-4-nitropenyl)-3-(4-nitropenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, sodium salt
Solubility:	water, DMSO
Quality control:	NMR ¹H and HPLC-MS (95+%)
Storage conditions:	24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS:	下載
	產品規格

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.