VU (5-Vinyl-uridine)
| 貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
|---|---|---|---|---|
| 3517-10mg | 10 mg | $125 | 現貨 | |
| 3517-50mg | 50 mg |
$449
|
現貨 |
5-Vinyl-uridine (VU) is a uridine derivative with a terminal alkene group that can be used for monitoring and investigating RNA transcription in cells instead of 5-ethynyl-uridine (EU).
VU is readily taken up by living cells and incorporated by RNA polymerases into de novo RNA instead of endogenous uridine, but not into DNA [1]. VU-labeled nascent cellular RNA can be detected quickly and with high sensitivity via inverse electron-demand Diels-Alder cycloaddition reaction (IEDDA) between the vinyl group and fluorescently or biotin-labeled tetrazines.
Labeled RNA can be detected with different cell transcriptional levels estimation methods, e.g., fluorescent microscopy or flow cytometry.
[1] Liu H.S. et al. A Nucleoside Derivative 5-Vinyluridine (VrU) for Imaging RNA in Cells and Animals. Bioconjug.Chem. 2019. 30(11). 2958-2966.
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| Appearance: | white solid |
| Molecular weight: | 270.24 |
| CAS number: | 55520-64-4 |
| Molecular formula: | C11H14N2O6 |
| Solubility: | DMSO, methanol |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | 下載 |
| 產品規格 |
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