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TAMRA maleimide, 6-isomer

TAMRA (TMR, Tetramethylrhodamine) Maleimides

貨號	數量	價格	交貨時間
18180	1 mg	$110	現貨
28180	5 mg	$260	現貨
48180	25 mg	$410	現貨
58180	50 mg	$695	現貨
68180	100 mg	$1190	現貨

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TAMRA (also known as TMR or tetramethylrhodamine) is a xanthene dye that has been used as a fluorescent label for decades. Xanthene dyes are available as two isomers (called 5- and 6-isomers) that have almost identical fluorescent properties but need to be separated to avoid doubling and smearing of labeled product peaks or bands during chromatography or electrophoresis. This is a pure 6-isomer of TAMRA maleimide, used to label proteins and peptides via thiol (SH) groups.

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建議協議

Maleimide Labeling of Proteins and Other Thiolated Biomolecules

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AF 430 maleimide

AF 430 is a green-yellow emitting fluorescent dye of the coumarin series. This maleimide derivative allows the labeling of thiolated biomolecules.

BDP 581/591 maleimide

Thiol reactive maleimide derivative of BDP 581/591 dye. The fluorophore is useful for a variety of application, including fluorescence polarization assays, and microscopy.

General properties

Appearance:	dark colored solid
Mass spec M+ increment:	551.2
Molecular weight:	552.58
Molecular formula:	C₃₁H₂₈N₄O₆
Solubility:	good in DMSO, DMF
Quality control:	NMR ¹H, HPLC-MS (95%)
Storage conditions:	Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS:	下載
	產品規格

Spectral properties

Excitation/absorption maximum, nm:	541
ε, L⋅mol⁻¹⋅cm⁻¹:	84000
Emission maximum, nm:	567
Fluorescence quantum yield:	0.1
CF₂₆₀:	0.32
CF₂₈₀:	0.19

產品引用

Li, H.; Li, X.; Chen, L.; Li, B.; Dong, H.; Liu, H.; Yang, X.; Ueda, H.; Dong, J. Quench-Release-Based Fluorescent Immunosensor for the Rapid Detection of Tumor Necrosis Factor α. ACS Omega, 2021, 6(46), 31009–31016. doi: 10.1021/acsomega.1c03941
Mendez, A.S.; Ly, M.; González-Sánchez, A.M.; Hartenian, E.; Ingolia, N.T.; Cate, J.H.; Glaunsinger, B.A. The N-terminal domain of SARS-CoV-2 nsp1 plays key roles in suppression of cellular gene expression and preservation of viral gene expression. Cell Reports, 2021, 37(3), 109841. doi: 10.1016/j.celrep.2021.109841
Ast, J.; Arvaniti, A.; Fine, N. H. F.; Nasteska, D.; Ashford, F. B.; Stamataki, Z.; Koszegi, Z.; Bacon, A.; Jones, B. J.; Lucey, M. A.; Sasaki, S.; Brierley, D. I.; Hastoy, B.; Tomas, A.; D’Agostino, G.; Reimann, F.; Lynn, F. C.; Reissaus, C. A.; Linnemann, A. K.; D’Este, E.; Calebiro, D.; Trapp, S.; Johnsson, K.; Podewin, T.; Broichhagen, J.; Hodson, D. J. Super-Resolution Microscopy Compatible Fluorescent Probes Reveal Endogenous Glucagon-like Peptide-1 Receptor Distribution and Dynamics. Nature Communications, 2020, 11(1), 467. doi: 10.1038/s41467-020-14309-w
Sagert, L.; Hennig, F.; Thomas, C.; Tampé, R. A loop structure allows TAPBPR to exert its dual function as MHC I chaperone and peptide editor. eLife, 2020, 9, e55326. doi: 10.7554/eLife.55326