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sulfo-Cyanine3 hydrazide

貨號 數量 價格 交貨時間
11370 1 mg $125 現貨
21370 5 mg $325 現貨
41370 25 mg $850 現貨
51370 50 mg $1490 現貨
61370 100 mg $1990 現貨
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sulfo-Cyanine3 is a sulfated derivative of Cyanine3 dye, well soluble in water because of two negatively charged sulfo groups in its structure. By its spectral characteristics, this yellow-orange fluorescent dye is an analog of Cy™3.

Hydrazides efficiently react with aldehydes and ketones resulting in hydrazones, so this compound can be used for conjugation with carbonyl derivatives of biomolecules.

The reaction runs in aqueous conditions, which is important when working with antibodies and proteins. Cys-diol groups in sugars in glycosylated proteins and antibodies can be oxidized into dialdehydes, and cysteine in proteins can be converted with enzymes to formyl glycerol (i. e. reactive groups for conjugation with sulfo-Cyanine3 hydrazide). Carboxyl groups of aspartic and glutamic acids in proteins and peptides can be also conjugated with sulfo-Cyanine3 hydrazide in the presence of activating agents: carbodiimide (EDAC) or methyl morpholine (DMTMM) derivatives.

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AF 488 酰肼 (AF 488 hydrazide)

AF 488 酰肼是一種羰基活性染料,可與醛和酮結合。

sulfo-Cyanine5.5 amine

sulfo-Cyanine5.5 is a far-red/NIR fluorophore possessing high hydrophilicity and aqueous solubility. This is an amine derivative that is reactive toward electrophiles. It can also be used for enzymatic transamination labeling.

AF 488 NHS 酯 (AF 488 NHS ester)

AF 488 NHS 酯,一種明亮、光穩定、親水性的綠色通道熒光染料。

General properties

Appearance: dark red solid
Molecular weight: 630.78
CAS number: 2144762-62-7
Molecular formula: C30H38N4O7S2
IUPAC name: 3H-​Indolium, 2-​[3-​[1-​(6-​hydrazinyl-​6-​oxohexyl)​-​1,​3-​dihydro-​3,​3-​dimethyl-​5-​sulfo-​2H-​indol-​2-​ylidene]​-​1-​propen-​1-​yl]​-​1,​3,​3-​trimethyl-​5-​sulfo-​, inner salt
Solubility: soluble in water, DMF, DMSO
Quality control: NMR 1H, HPLC-MS (90%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: 下載
產品規格

Spectral properties

Excitation/absorption maximum, nm: 548
ε, L⋅mol−1⋅cm−1: 162000
Emission maximum, nm: 563
Fluorescence quantum yield: 0.1
CF260: 0.03
CF280: 0.06
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