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sulfo-Cyanine3 DBCO

Cycloalkynes Copper-free click chemistry sulfo-Cyanine3
貨號 數量 價格 交貨時間
113F0 1 mg $125 現貨
213F0 5 mg $325 現貨
413F0 25 mg $850 現貨
513F0 50 mg $1490 現貨
613F0 100 mg $1990 現貨
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sulfo-Cyanine3 DBCO is a water-soluble fluorescent dye with cycloalkyne group for copper-free click chemistry.

DBCO (dibenzocyclooctyne) is a cyclooctyne with very high reactivity towards azides, still possessing good stability.

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sulfo-Cyanine3 carboxylic acid

sulfo-Cyanine3 free unactivated monofunctional carboxylic acid, water-soluble dye.

AmdU (5-Azidomethyl-2'-deoxyuridine)

Azidomethyldeoxyuridine (AmdU) is an azide-contained nucleoside that is incorporated into nascent DNA by cellular polymerases. The azide groups attached to DNA this way can be subsequently modified with alkynes or cycloalkynes via copper-catalyzed or strain-promoted click reaction.

花青3B DBCO (Cyanine3B DBCO)

二苯並環辛炔（DBCO、ADIBO）含有 Cyanine3B 螢光團，用於標記活細胞、整個生物體和無生命樣品內的疊氮標記的生物分子。

General properties

Appearance: red solid
Mass spec M+ increment: 916.4
Molecular weight: 955.23
Molecular formula: C51H55N4KO8S2
Solubility: soluble in water (0.11 M = 11 g/L), DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: 下載
產品規格

Spectral properties

Excitation/absorption maximum, nm: 548
ε, L⋅mol−1⋅cm−1: 162000
Emission maximum, nm: 563
Fluorescence quantum yield: 0.1

產品引用

  1. Michielsen, C. M. S.; van Aalen, E. A.; Merkx, M. Ratiometric Bioluminescent Zinc Sensor Proteins to Quantify Serum and Intracellular Free Zn2+. ACS Chem. Biol., 2022, 17(6), 1567–1576. doi: 10.1021/acschembio.2c00227
  2. Tugel, U.; Casas, M.G.; Wiltschi, B. Site-Specific Incorporation of Non-canonical Amino Acids by Amber Stop Codon Suppression in Escherichia coli. Peptide and Protein Engineering (Springer Protocols Handbooks), 2020, 267–281. doi: 10.1007/978-1-0716-0720-6_14
  3. Desai, B.J.; Gonzalez, R.L. Multiplexed genomic encoding of non-canonical amino acids for labeling large complexes. Nature Chemical Biology, 2020, 16, 1129–1135. doi: 10.1038/s41589-020-0599-5
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