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Ribo488 RNA Quantification Reagent

PCR and qPCR Nucleic acid quantification 螢光測定法

貨號	數量	價格	交貨時間
11510	100 uL	–	現貨
21510	250 uL	$198	現貨
41510	1 mL	$639	現貨
51510	1.25 mL	$729	現貨

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Ribo488 RNA Quantification Reagent is an ultra-sensitive dye. It enables the detection of the RNA/dye complex fluorescence with high selectivity and in a wide range of RNA concentrations with a linear dependence. Ribo488 RNA dye exhibits bright green fluorescence when binding to nucleic acids, with excitation/emission peaks at 500 and 525 nm, respectively.

Fluorescence staining of RNA with Ribo488 can be used in applications that require RNA preparation for microarray samples, real-time reverse transcription PCR (RT-PCR) assays, differential display PCR, Northern blot, S1 nuclease assays, RNase protection assays, and to construct cDNA libraries.

Reagent is supplied as 400 µM solution of dye in anhydrous dimethylsulfoxide (DMSO).

The DMSO stock solution should be stored at −20 °C. The working solution of the Ribo488 RNA Quantification Reagent must be protected from light, and be used within a few hours of its preparation.

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General properties

Appearance:	orange solution
Quality control:	NMR ¹H, HPLC-MS (95%), functional testing
Storage conditions:	Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS:	下載
	產品規格

Spectral properties

Excitation/absorption maximum, nm:	493
Emission maximum, nm:	525
Fluorescence quantum yield:	0.53

Product is offered and sold for research purposes only. Product is not tested for safety and efficacy in food, drug, medical device, cosmetic, no express or implied authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, for humans or animals or for commercial purposes.