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Copper(II)-THPTA catalytic buffer, 1.5x

Click chemistry

貨號	數量	價格	交貨時間
B5150	1 mL	$30	現貨
K5150	10 mL	$120	現貨
S5150	50 mL	$490	現貨

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Catalytic buffer is suitable for coupling of azide- or alkyne-modified protein with alkyne- or azide- bearing dye via Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC). THPTA ligand accelerates the reaction rate due to the stabilization of a catalytically active Cu (I). Moreover, the presence of water-soluble THPTA allows the protein labeling to be run in aqueous solution and, by chelating free copper, minimizes the generation of ROS (reactive oxygen species) and undesired damage of proteins. Aminoguanidine prevents connections of reactive aldehydes, which are products of dehydroascorbate hydrolysis, with arginine, N-terminal cysteine, and lysine side-chains. Ready-to-use 1.5x buffer provides all the necessary reagents to perform the CuAAC reaction, except a reagent for the reduction of Cu (II) into catalytically active Cu (I). As a reducing agent we recommend ascorbic acid.

Buffer composition: Cu (II), triethylammonium acetate buffer pH 6.8, THPTA ligand, aminoguanidine

建議協議

Conjugation of Alkyne- or Azide-modified Proteins with Dye Azides or Alkynes

計算機

解决方案计算器

General properties

Storage conditions:	Storage: 12 months after receival at +4 or -20°C in the dark. Transportation: at room temperature for up to 3 weeks.
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