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DusQ 3 CPG 500

DusQ 3 CPG modifier solid supports
貨號 數量 價格 交貨時間
1618-100mg 100 mg –   現貨
1618-1g 1 g $990 現貨
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DusQ 3 is a dark quencher for far red and near infrared fluorophores, especially suitable for Cyanine5.5.

The dye can be attached to 3'-terminus of oligonucleotides by means of automated solid phase synthesis using this support. The dye is sensitive to nucleophilic reagents, and therefore must be carefully deprotected. We recommend the use of ultramild, phenoxyacetyl protected monomers. As an alternative, post-synthetic labeling using NHS ester of the quencher can be used.

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六角疊氮化物, 6-異構體 (HEX azide, 6-isomer)

綠色-黃色發射 HEX 染料的疊氮化物，通過 [3+2]-偶極環加成與寡核苷酸偶合。

JOE phosphoramidite, 5-isomer

JOE phosphoramidite is a xanthene dye phosphoramidite used in oligo synthesis. Its absorption and emission spectra are located between the FAM and TAMRA channels.

FAM 亞磷酰胺，6-異構體 (FAM phosphoramidite, 6-isomer)

單一構體 6-FAM 亞磷酰胺，用於寡核苷酸的修飾。

General properties

Appearance: dark blue beads
Quality control: 1H NMR of bound reagent, coupling testing with HPLC-MS
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
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產品規格

Spectral properties

Excitation/absorption maximum, nm: 657
ε, L⋅mol−1⋅cm−1: 45500

Oligo synthesis details

Pore size, Å: 500
Typical loading, umol/g: 50-80
Coupling conditions: standard, ultramild phosphoramidites
Cleavage conditions: 2 h / rt, 30% aq. ammonia (ultramild protective groups)
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