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JOE azide, 5-isomer

Azides JOE Click chemistry
貨號 數量 價格 交貨時間
A1130 1 mg $110 現貨
B1130 5 mg $210 現貨
C1130 10 mg $310 現貨
D1130 25 mg $410 現貨
E1130 50 mg $695 現貨
F1130 100 mg $1190 現貨
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JOE is a fluorescent dye with the emission in yellow region of the spectrum. The fluorophore matches channel of HEX dye, another chlorinated fluorescein.

JOE dye is often used in qPCR; oligo labeling can be achieved using сlick chemistry. This is an azide derivative of JOE for сlick chemistry, pure 5-isomer.

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JOE alkyne, 6-isomer

JOE dye alkyne derivative for copper-catalyzed click chemistry reaction with azides. JOE dye is widely used for nucleic acid labeling.

TAMRA phosphoramidite, 5-isomer

TAMRA phosphoramidite for synthesis of 5’-labeled oligonucleotides. Pure 5-isomer.
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Cyanine3.5 azide

Cyanine3.5 azide for biomolecule labeling through click chemistry.

General properties

Appearance: red solid
Mass spec M+ increment: 586.1
Molecular weight: 587.37
CAS number: 1422178-11-7
Molecular formula: C26H20N4Cl2O8
Solubility: good in DMF, DMSO
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
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產品規格

Spectral properties

Excitation/absorption maximum, nm: 533
ε, L⋅mol−1⋅cm−1: 75000
Emission maximum, nm: 554
Fluorescence quantum yield: 0.61
CF260: 0.36
CF280: 0.28

產品引用

  1. Taskova, M.; Astakhova, K. Fluorescent Oligonucleotides with Bis(prop-2-yn-1-yloxy)butane-1,3-diol Scaffold Rapidly Detect Disease-Associated Nucleic Acids. Bioconjugate Chemistry, 2019, 30(12), 3007–3012. doi: 10.1021/acs.bioconjchem.9b00746
  2. Nåbo, L.J.; Madsen, C.S.; Jensen, K.J.; Kongsted, J.; Astakhova, K. Ultramild Protein-Mediated Click Chemistry Creates Efficient Oligonucleotide Probes for Targeting and Detecting Nucleic Acids. ChemBioChem, 2015, 16(8), 1163–1167. doi: 10.1002/cbic.201500145
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