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DusQ® 21 CPG 500

貨號 數量 價格 交貨時間
3151-100mg 100 mg –   現貨
3151-1g 1 g $990 現貨
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DusQ® 21 is a quencher for the dyes in far red region of the spectrum. The dye is a xanthene derivative, unlike DusQ 1 or DusQ 2, that are azo dyes. It has a high extinction coefficient, and provides an outstanding quenching efficiency.

The controlled pore glass solid support allows to synthesize oligonucleotides carrying the dye moiety on 3’-terminus.

We recommend the use of ultramild, phenoxyacetyl protected monomers. As an alternative, post-synthetic labeling using NHS ester of DusQ 21 quencher can be used.

Usage

Coupling: Standard conditions identical to normal nucleobases.

Deprotection: 2 hours at room temperature using concentrated ammonia or potassium carbonate K2CO3 (50 mM solution in methanol).

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JOE 疊氮化物,5-異構體 (JOE azide, 5-isomer)

JOE 是一種氯代螢光素染料,常用於寡核苷酸的 HEX 通道。它的疏水性比 HEX 弱。是一種疊氮衍生物,適用於點擊化學。

FAM 亞磷酰胺,6-異構體 (FAM phosphoramidite, 6-isomer)

單一構體 6-FAM 亞磷酰胺,用於寡核苷酸的修飾。

炔烴 CPG 改性劑 500 (Alkyne CPG modifier 500)

為點擊化學合成帶有炔基的 3'-修飾寡核苷酸提供了堅實的支持。

General properties

Appearance: dark blue beads
Quality control: 1H NMR of bound reagent, coupling testing with HPLC-MS
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
MSDS: 下載
產品規格

Spectral properties

Excitation/absorption maximum, nm: 656
ε, L⋅mol−1⋅cm−1: 72800

Oligo synthesis details

Pore size, Å: 500
Typical loading, umol/g: 50−80
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