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Cyanine3 tetrazine

Tetrazines Cyanine3 Copper-free click chemistry
貨號 數量 價格 交貨時間
110E0 1 mg $125 現貨
210E0 5 mg $260 現貨
410E0 25 mg $510 現貨
510E0 50 mg $895 現貨
610E0 100 mg $1490 現貨
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Cyanine3 tetrazine is a fluorescent dye with tetrazine moiety for a conjugation reaction called TCO (trans-cyclooctene) ligation. Actually, tetrazines react not only with trans-cyclooctenes, but also with cyclopropenes and strained non-benzo-fused cycloalkynes.

Cyanine3 is a moderately hydrophobic dye, enabling labeling in non-aqueous environment as well as in aqueous media containing some organic co-solvent like acetonitrile or DMSO. For reactions in purely aqueous media, a sulfonated version of this reagent is available.

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Cyanine7 NHS 酯 (Cyanine7 NHS ester)

近紅外染料 Cyanine7 的胺反應活性 NHS 酯。

Cyanine3疊氮化物 (Cyanine3 azide)

用於 сlick сhemistry 的 Cyanine3 螢光染料的疊氮衍生物。

磺基-Cyanine7 炔烴 (sulfo-Cyanine7 alkyne)

一種用於銅催化與疊氮化物環加成的磺化近紅外染料炔烴。

General properties

Appearance: dark red solid
Mass spec M+ increment: 611.4
Molecular weight: 727.64
CAS number: 2183473-57-4
Molecular formula: C40H46N7BF4O
Solubility: good in DCM, DMSO, DMF
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: 下載
產品規格

Spectral properties

Excitation/absorption maximum, nm: 555
ε, L⋅mol−1⋅cm−1: 150000
Emission maximum, nm: 570
Fluorescence quantum yield: 0.31
CF260: 0.04
CF280: 0.09

產品引用

  1. Bai, M.; Cao, X.; Huang, W.; Chen, F.; Zhao, Y.; Xue, J.; Li, Y.; Cheng, Y.; Zhang, L.; Zhao, Y. Visualizing Newly Synthesized RNA by Bioorthogonal Labeling-primed DNA Amplification. Analytical Chemistry, 2020, 92(12), 8444–8449. doi: 10.1021/acs.analchem.0c01161
  2. Moutsiopoulou, A.; Broyles, D.; Joda, H.; Dikici, E.; Kaur, A.; Kaifer, A.E.; Daunert, S.; Deo, S.K. Bioluminescent Protein-Inhibitor Pair in the Design of a Molecular Aptamer Beacon Biosensing System. Analytical Chemistry, 2020, 92(11), 7393–7398. doi: 10.1021/acs.analchem.0c00518
  3. Chen, F.; Bai, M.; Cao, X.; Zhao, Y.; Xue, J.; Zhao, Y. Click-encoded rolling FISH for visualizing single-cell RNA polyadenylation and structures. Nucleic Acids Research, 2019, 47(22), e145. doi: 10.1093/nar/gkz852
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