Bis-ANS, protein conformation probe
貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
---|---|---|---|---|
4381-5mg | 5 mg | $159 | 現貨 | |
4381-25mg | 25 mg | $397 | 現貨 |
Bis-ANS is a high-affinity fluorescent probe for nonpolar cavities in proteins. Its hydrophobic phenyl and naphthyl rings interact noncovalently with proteins and protein degradation products.
As with other anilinonaphthalene sulfonates (ANS), bis-ANS is essentially nonfluorescent in water but becomes noticeably fluorescent in a nonpolar environment. When free, bis-ANS has an excitation maximum at 390 nm and an emission maximum at 523 nm but undergoes a blue shift with an increase in fluorescence intensity when bound to protein.
Bis-ANS is frequently used to monitor the formation of protein aggregates and indicate protein folding and conformational changes. The dye is also used to detect Aβ fibers.
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Appearance: | light yellow-green crystals |
Molecular weight: | 672.87 |
CAS number: | 65664-81-5 |
Molecular formula: | C32H22K2N2O6S2 |
IUPAC name: | dipotassium;8-anilino-5-(4-anilino-5-sulfonatonaphthalen-1-yl)naphthalene-1-sulfonate |
Solubility: | DMF: 30 mg/ml, DMSO: 30 mg/ml, ethanol: slightly soluble, PBS (pH 7.2): 5 mg/ml |
Quality control: | NMR 1H and HPLC-MS (95+%) |
Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
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