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Biotin-PEG4-NHS ester

Biotinylation NHS酯類 Crosslinking

貨號	數量	價格	交貨時間	購買此產品
2893-100mg	100 mg	$145	現貨
2893-1g	1 g	$590	現貨

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Biotin-PEG4-NHS ester is an activated ester for simple and efficient biotin labeling of antibodies, proteins, and other primary amine-containing biomolecules. The NHS ester group reacts efficiently with amino groups by the nucleophilic attack, forming an amide bond and releasing N-Hydroxysuccinimide. Biotin-labeled compounds can then be linked to avidin or streptavidin for further purification or detection. The biotin group is relatively small and doesn’t affect the biological activity of biotinylated proteins.

This reagent features a long PEG4-linker that separates the biotin residue from the target molecule, ensuring efficient binding to avidin or streptavidin. The linker also increases the compound’s water solubility, thereby facilitating bioconjugation. Antibodies labeled with Biotin-PEG4-NHS ester exhibit less aggregation when stored in solution than those labeled with reagents with only hydrocarbon spacers.

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建議協議

NHS Ester Labeling of Biomolecules Containing Primary Amino Groups

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蛋白質標記計算器

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DusQ 1 NHS ester

Functionalized DusQ 1 quencher derivative with quenching range from 480 - 580 nm for labeling of amine-containing molecules.

General properties

Appearance:	white to off-beige powder
Molecular weight:	588.68
CAS number:	459426-22-3
Molecular formula:	C₂₅H₄₀N₄O₁₀S
Solubility:	water, DMSO, DMF, DCM
Quality control:	NMR ¹H and HPLC-MS (95+%)
Storage conditions:	12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS:	下載
	產品規格

產品引用

Zhang, L.; Yang, C.; Li, Y.; Niu, S.; Liang, X.; Zhang, Z.; Luo, Q.; Luo, H. Dynamic Changes in the Levels of Amyloid-β 42 Species in the Brain and Periphery of APP/PS1 Mice and Their Significance for Alzheimer’s Disease. Frontiers in Molecular Neuroscience, 2021, 14, 723317. doi: 10.3389/fnmol.2021.723317

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.