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Biotin-PEG3-azide

Azides Biotinylation Click chemistry
貨號 數量 價格 交貨時間
C3730 10 mg $125 現貨
D3730 25 mg $220 現貨
E3730 50 mg $325 現貨
F3730 100 mg $450 現貨
K3730 1 g $1420 現貨
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Biotin-PEG3-azide is a click chemistry biotinylation tool. This reagent allows the labeling of alkynylated molecules (such as DNA, oligonucleotides, and proteins) with biotin via copper-catalyzed or copper-free click reaction. Biotin-labeled biomolecules can be bound to avidin or streptavidin for further purification and detection.

This structure of biotin azide features a long hydrophilic PEG3 linker that separates biotin residue from the target molecule for efficient binding with streptavidin. Its linker also enhances aqueous solubility to facilitate conjugation.

The azide can be conjugated with various molecules using our recommended protocol.

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Copper(II)-THPTA catalytic buffer, 1.5x

Ready-to-use catalytic buffer containing сopper(II) and water-soluble THPTA ligand. It is suitable for click chemistry modification of proteins.

THPTA配體

THPTA 是 Cu(I) 催化點擊化學的水溶性配體。該配體使銅穩定在 Cu(I) 氧化態。由於水溶性高，與該配體的反應不需要有機共溶劑。

General properties

Appearance: colorless solid
Molecular weight: 400.50
CAS number: 1910803-72-3
Molecular formula: C16H28N6O4S
Solubility: soluble in DMF, DMSO, moderately soluble in water
Quality control: NMR 1H (95%) and 13C, TLC, functional testing
Storage conditions: Storage: 24 months after receival at -20°C. Transportation: at room temperature for up to 3 weeks.
MSDS: 下載
產品規格

產品引用

  1. Liang, J.; Han, J.; Gao, X.; Jia, H.; Li, R.; Tse, E. C. M.; Li, Y. Clickable APEX2 Probes for Enhanced RNA Proximity Labeling in Live Cells. Anal. Chem., 2024, 96(2), 685–693. doi: 10.1021/acs.analchem.3c03614
  2. Edr, A.; Wrobel, D.; Krupková, A.; Šťastná, L. Č.; Cuřínová, P.; Novák, A.; Malý, J.; Kalasová, J.; Malý, J.; Malý, M.; Strašák, T. Adaptive Synthesis of Functional Amphiphilic Dendrons as a Novel Approach to Artificial Supramolecular Objects. International Journal of Molecular Sciences, 2022, 23(4), 2114. doi: 10.3390/ijms23042114
  3. Shen, J.Z.; Qiu, Z.; Wu, Q.; Finlay, D.; Garcia, G.; Sun, D.; Rantala, J.; Barshop, W.; Hope, J.L.; Gimple, R.C.; Sangfelt, O.; Bradley, L.M.; Wohlschlegel, J.; Rich, J.N.; Spruck, C. FBXO44 promotes DNA replication-coupled repetitive element silencing in cancer cells. Cell, 2021, 184(2), 352–369.e23. doi: 10.1016/j.cell.2020.11.042
  4. Zeng, Q.; Deng, H.; Li, Y.; Fan, T.; Liu, Y.; Tang, S.; Wei, W.; Liu, X.; Guo, X.; Jiang, J.; Wang, Y.; Song, D. Berberine Directly Targets the NEK7 Protein to Block the NEK7–NLRP3 Interaction and Exert Anti-inflammatory Activity. Journal of Medicinal Chemistry, 2021, 64(1), 768–781. doi: 10.1021/acs.jmedchem.0c01743
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