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Alkyne dT phosphoramidite

Alkynes Oligonucleotide synthesis Click chemistry Phosphoramidites
貨號 數量 價格 交貨時間
1897-100mg 100 mg $330 現貨
1897-1g 1 g $2590 現貨
1897-5g 5 g $9900 現貨
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This reagent is designed for the synthesis of oligonucleotides bearing triple bond (alkyne) in the middle of the chain. Alkyne is attached to deoxyuridine fragment, and it does not interfere with the hybridization to the complementary strand. This allows to prepare modified oligonucleotide probes bearing fluorescent dyes, quenchers, and other fragments attached to the middle of the chain, by virtue of copper catalyzed Click chemistry reaction with various azides.

This phosphoramidite is solid, so it is easy to dispense and handle. It is compatible with the standard deblocking conditions.

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DusQ 1 CPG 1000

Controlled pore glass (CPG) 1000 solid support for synthesis of oligonucleotides with 3’-terminal DusQ1 quencher. Compatible with standard deblocking conditions.
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R6G azide, 5-isomer

Rhodamine 6G is a xanthene dye that has been used for DNA sequencing and qPCR. This azide derivative is used for the attachment of the dye to DNA and other molecules by means of click chemistry.

TAMRA phosphoramidite, 5-isomer

TAMRA phosphoramidite for synthesis of 5’-labeled oligonucleotides. Pure 5-isomer.
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General properties

Appearance: off white solid
Mass spec M+ increment: 437.1
Molecular weight: 877.96
Molecular formula: C48H56N5O9P
Solubility: good in acetonitrile, DCM
Quality control: NMR 1H, NMR 31P, HPLC-MS
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
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產品規格

Oligo synthesis details

Diluent: acetonitrile
Coupling conditions: standard coupling, identical to normal nucleobases
Cleavage conditions: ammonia, 2 h at room temperature
Deprotection conditions: identical to protected nucleobases
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