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AF 568 DBCO

AF 568 Cycloalkynes Copper-free click chemistry
貨號 數量 價格 交貨時間
1158F0 1 mg $140 現貨
2158F0 5 mg $450 現貨
4158F0 25 mg $1460 現貨
5158F0 50 mg $1890 現貨
6158F0 100 mg $2990 現貨
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Dibenzocyclooctyne (DBCO, DBCO, ADIBO) is one of the most reactive cycloalkynes for copper-free click reaction (SPAAC, strain-promoted azide-alkyne cycloaddition). The rate of interaction of DBCO with azides is significantly higher than that of other cyclooctynes, as well as Cu-catalyzed click reaction (CuAAC). Unlike other cyclooctynes, DBCO does not interact with tetrazines, which makes it possible to use it in bioorthogonal reactions together with trans-cyclooctenes and tetrazines.

AF 568 is a bright, photostable, and hydrophilic fluorophore that emits in the orange channel. The absorption maximum is 572 nm. The emission maximum is 598 nm.

AF 568 DBCO allows fluorescent labeling of azide-containing biomolecules inside living cells and whole organisms without the negative effect of copper ions on them, and inanimate samples.

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AmdU (5-Azidomethyl-2'-deoxyuridine)

Azidomethyldeoxyuridine (AmdU) is an azide-contained nucleoside that is incorporated into nascent DNA by cellular polymerases. The azide groups attached to DNA this way can be subsequently modified with alkynes or cycloalkynes via copper-catalyzed or strain-promoted click reaction.

sulfo-Cyanine3 NHS酯 (sulfo-Cyanine3 NHS ester)

水溶性磺基-Cyanine3琥珀醯亞胺酯 (SE) 活化酯，用於氨基生物分子標記。

TAMRA DBCO, 5-isomer

Cyclooctyne derivative of tetramethylrhodamine (TMR, TAMRA) for copper-free click chemistry conjugation with azides. Pure 5-isomer.

General properties

Appearance: dark violet solid
Molecular weight: 1197.53
Molecular formula: C66H80N6O11S2
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light.
MSDS: 下載
產品規格

Spectral properties

Excitation/absorption maximum, nm: 572
ε, L⋅mol−1⋅cm−1: 94238
Emission maximum, nm: 598
Fluorescence quantum yield: 0.912
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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