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AF 488 carboxylic acid

AF 488 Carboxylic acids

貨號	數量	價格	交貨時間
11890	1 mg	$125	現貨
21890	5 mg	$325	現貨
41890	25 mg	$950	現貨
51890	50 mg	$1625	現貨
61890	100 mg	$2090	現貨

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AF 488 is a bright green-fluorescent dye that is commonly used in microscopy and cell assays because of its photostability. AF 488 can be used with DAPI and is well suited to multiplex assay. AF 488 has high quantum yield and stable fluorescence within the pH range from 4 to 10.

AF 488 carboxylic acid is a non-reactive AF 488 form that can be used as a reference standard in experiments where AF 488 conjugates are used. The carboxylic acid can be also used for the synthesis of activated esters [such as sulfo-NHS, TFP (2,3,5,6-tetrafluorophenol) and STP (4-sulfo-2,3,5,6-tetrafluorophenol)] or modified with hydrazines, hydroxylamines, or amines in aqueous solutions using water-soluble carbodiimides. Thus, this derivative can be conjugated to molecules that contain amino groups, such as proteins, antibodies, and peptides. Therefore, AF 488 carboxylic acid is used during solid-phase peptide synthesis for peptide modification in situ in the presence of activating agents such as HATU.

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General properties

Appearance:	orange crystals
Molecular weight:	648.75
Molecular formula:	C₂₁H₁₁K₃N₂O₁₁S₂
IUPAC name:	4-(6-amino-3-iminio-4,5-disulfonato-3H-xanthen-9-yl)isophthalate
Solubility:	good in DMSO, DMF
Quality control:	NMR ¹H, HPLC-MS (95%)
Storage conditions:	Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS:	下載
	產品規格

Spectral properties

Excitation/absorption maximum, nm:	495
ε, L⋅mol⁻¹⋅cm⁻¹:	71800
Emission maximum, nm:	519
Fluorescence quantum yield:	0.91
CF₂₆₀:	0.16
CF₂₈₀:	0.10