Universal CPG type II, 500A
| 貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
|---|---|---|---|---|
| 3219-1g | 1 g | $45 | 現貨 | |
| 3219-10g | 10 g |
$410
|
現貨 | |
| 3219-100g | 100 g | 請查詢 | 21 天 |
The Universal CPG type II, 500A is used for oligonucleotides synthesis to increase rate of dephosphorylation of the 3' end oligonucleotide during deblocking due to a rigid bicyclic molecule on the support.
The Universal CPG type II, 500A is suitable for use in harsh conditions and makes cleavage and deprotection with anhydrous ammonia gas-phase, ammonium hydroxide/methylamine (AMA) mixture or other basic reagents faster compared to universal Controlled Pore Glass (CPG) supports. Pore size of 500 Å is recommended for the synthesis of oligonucleotides up to 120 bases. For oligos up to 120 bases universal support 1000 Å can be used.
Usage
Coupling: Standard conditions for universal CPG.
Deprotection: 2 hours at 80
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R6G phosphoramidite, 6-isomer
Phosphoramidite for synthesis of oligonucleotides labeled at the 5'-terminus with rhodamine 6G (R6G). Pure 6-isomer.
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Universal CPG type I, 500A
This universal support is intended for the automated synthesis of all types of oligonucleotides. Pore size of 500 Å is recommended for the synthesis of oligonucleotides up to 50 bases in length.六角疊氮化物, 6-異構體 (HEX azide, 6-isomer)
綠色-黃色發射 HEX 染料的疊氮化物，通過 [3+2]-偶極環加成與寡核苷酸偶合。General properties
| Appearance: | white powder |
| Quality control: | loading measurement, functional testing in oligo synthesis. |
| Storage conditions: | 24 months after receival at −20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | 下載 |
| 產品規格 |
Oligo synthesis details
| Pore size, Å: | 500 |
| Typical loading, umol/g: | 50−80 |
| Coupling conditions: | standard coupling, identical to normal nucleobases |
| Cleavage conditions: | ammonium hydroxide 2 hours at 80 °C or AMA mixture, ammonium hydroxide - 40% methylamine (1:1), 15 minutes at 65 °C |
| Deprotection conditions: | identical to protected nucleobases |
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