sulfo-Cyanine5 hydrazide
| 貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
|---|---|---|---|---|
| 13370 | 1 mg | $125 | 現貨 | |
| 23370 | 5 mg | $325 | 現貨 | |
| 43370 | 25 mg |
$850
|
現貨 | |
| 53370 | 50 mg |
$1490
|
現貨 | |
| 63370 | 100 mg |
$1990
|
現貨 |
sulfo-Cyanine5 is a sulfated derivative of Cyanine5 dye, well soluble in water because of two negatively charged sulfo groups in its structure. By its spectral characteristics, this far-red fluorescent dye is an analog of Cy™5.
Hydrazides efficiently react with aldehydes and ketones resulting in hydrazones, so this compound can be used for conjugation with carbonyl derivatives of biomolecules.
The reaction runs in aqueous conditions, which is important when working with antibodies and proteins. Cys-diol groups in sugars in glycosylated proteins and antibodies can be oxidized into dialdehydes, and cysteine in proteins can be converted with enzymes to formyl glycerol (i. e. reactive groups for conjugation with sulfo-Cyanine5 hydrazide). Carboxyl groups of aspartic and glutamic acids in proteins and peptides can be also conjugated with sulfo-Cyanine5 hydrazide in the presence of activating agents: carbodiimide (EDAC) or methyl morpholine (DMTMM) derivatives.
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磺基-Cyanine5 NHS 酯 (sulfo-Cyanine5 NHS ester)
水溶性磺基-Cyanine5 琥珀酰亞胺酯，用於在水相中標記各種含胺分子。磺基-Cyanine3馬來酰亞胺
磺基-Cyanine3的水溶性硫醇反應衍生物，一種Cy3 類似物。FAM 酰肼，5-異構體 (FAM hydrazide, 5-isomer)
FAM 酰肼是一種羰基反應性熒光素衍生物。純5-異構體。可以用羰基的氧化蛋白質、糖類和許多小分子。General properties
| Appearance: | dark blue solid |
| Molecular weight: | 656.83 |
| CAS number: | 2055138-61-7 |
| Molecular formula: | C32H40N4O7S2 |
| Solubility: | very good in water, good in DMF and DMSO |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. Avoid prolonged exposure to light. |
| MSDS: | 下載 |
| 產品規格 |
Spectral properties
| Excitation/absorption maximum, nm: | 646 |
| ε, L⋅mol−1⋅cm−1: | 271000 |
| Emission maximum, nm: | 662 |
| Fluorescence quantum yield: | 0.28 |
| CF260: | 0.04 |
| CF280: | 0.04 |
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