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sulfo-Cyanine5 dUTP

PCR and qPCR Triphosphates sulfo-Cyanine5
貨號 數量 價格 交貨時間
1510-50nmol 50 nmol $270 現貨
1510-250nmol 250 nmol $990 現貨
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sulfo-Cyanine5 dUTP (deoxyuridine triphosphate) is a modified nucleoside triphosphate for the enzymatic labeling of DNA. The triphosphate is an analog of Cy5-dUTP. However, unlike aminoallyl dUTP directly modified with dye, this triphosphate contains a longer linker arm that increases the efficiency of its incorporation by usual polymerases, like Taq polymerase.

sulfo-Cyanine5 is a bright, far-red emitting dye for the Cy5 channel.

The triphosphate is supplied in the lyophilized ready-to-use form of lithium salt.

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General properties

Appearance: blue solid / solution
Molecular weight: 1275.89
Molecular formula: C49H66N6Li4O22P3S2
Solubility: good in water
Quality control: HPLC-MS (95%), testing in enzymatic reaction
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light and excessive freeze-thaw cycles.
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產品規格

Spectral properties

Excitation/absorption maximum, nm: 646
ε, L⋅mol−1⋅cm−1: 271000
Emission maximum, nm: 662
Fluorescence quantum yield: 0.28
CF260: 0.04
CF280: 0.04

產品引用

  1. Emelyanova, M.; Pokataev, I.; Shashkov, I.; Kopantseva, E.; Lyadov, V.; Heydarov, R.; Mikhailovich, V. TYMS 3′-UTR Polymorphism: A Novel Association with FOLFIRINOX-Induced Neurotoxicity in Pancreatic Cancer Patients. Pharmaceutics, 2022, 14(1), 77. doi: 10.3390/pharmaceutics14010077
  2. Shaskolskiy, B.; Kandinov, I.; Kravtsov, D.; Vinokurova, A.; Gorshkova, S.; Filippova, M.; Kubanov, A.; Solomka, V.; Deryabin, D.; Dementieva, E.; Gryadunov, D. Hydrogel Droplet Microarray for Genotyping Antimicrobial Resistance Determinants in Neisseria gonorrhoeae Isolates. Polymers, 2021, 13(22), 3889. doi: 10.3390/polym13223889
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