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ProteOrange® Protein Gel Stain, 5000×

貨號 數量 價格 交貨時間
10210 100 uL –   現貨
40210 1 mL $285 現貨
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ProteOrange® is a protein-binding dye for staining protein gels, an analog of SYPRO Orange. The fluorophore is a styrene-like zwitterionic dye. It binds proteins selectively in the presence of nucleic acids, polysaccharides, and other molecules. It is also tolerant to SDS and, therefore, can be used for SDS-PAGE gel staining. The dye interacts with the SDS coat around proteins in the gel, giving more consistent staining between different types of proteins than CBB staining and never exhibiting negative staining. It also stains glycoproteins well.

Staining with ProteOrange is about an order of magnitude more sensitive than Coumassie dye staining and allows the detection of about 3 ng of protein per band (30 ng is the detection limit for Coumassie).

ProteOrange is supplied as a 5000x solution in DMSO. 1 mL of the solution is enough to staining around 200 minigels.

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PreciseGreen® dsDNA 定量試劑,200× (PreciseGreen dsDNA Quantification Reagent, 200×)

PreciseGreen® 是一種染料,可用於在RNA、ssDNA 和其他雜質存在的情況下對dsDNA進行精確熒光定量。該染料在熒光素通道中表現出明亮的熒光,在pg/mLng/mL 範圍內對 DNA 濃度提供線性響應。

BDP® TR tetrazine

BDP TR is a borondipyrromethene fluorophore for the ROX channel. The dye produces bright fluorescent emission. Tetrazines are reactive components for the conjugation with strained cycloalkenes.

DBCO-NHS 酯

偶氮二苯並環辛炔 (DBCO、ADIBO) 試劑用於應變促進的無銅點擊化學 (SPAAC)。該試劑含有 NHS 酯官能團,用於將環辛炔連接到各種分子上。

General properties

Appearance: orange solution
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: 下載
產品規格

Spectral properties

Excitation/absorption maximum, nm: 470
Emission maximum, nm: 570

產品引用

  1. Heesink, G.; van den Oetelaar, M.C.M.; Semerdzhiev, S.A.; Ottmann, C.; Brunsveld, L.; Blum, C.; Claessens, M.M.A.E. 14-3-3τ as a Modulator of Early α-Synuclein Multimerization and Amyloid Formation. ACS Chemical Neuroscience, 2024, 15(9), 1926-1936. doi: 10.1021/acschemneuro.4c00100
  2. Somsen, B. A.; Sijbesma, E.; Leysen, S.; Honzejkova, K.; Visser, E. J.; Cossar, P. J.; Obšil, T.; Brunsveld, L.; Ottmann, C. Molecular Basis and Dual Ligand Regulation of Tetrameric Estrogen Receptor α/14-3-3ζ Protein Complex. Journal of Biological Chemistry, 2023, 299(7), 104855. doi: 10.1016/j.jbc.2023.104855
  3. Vasilev, R.; Gunitseva, N.; Shebanova, R.; Korzhenkov, A.; Vlaskina, A.; Evteeva, M.; Polushkina, I.; Nikitchina, N.; Toshchakov, S.; Kamenski, P.; Patrushev, M.; Mazunin, I. Targeted Modification of Mammalian DNA by a Novel Type V Cas12a Endonuclease from Ruminococcus bromii. International Journal of Molecular Sciences, 2022, 23(16), 9289. doi: 10.3390/ijms23169289
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