ProteOrange Protein Gel Stain, 5000×
| 貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
|---|---|---|---|---|
| 10210 | 100 uL | – | 現貨 | |
| 40210 | 1 mL | $285 | 現貨 |
ProteOrange is a protein-binding dye for staining of protein gels, an analog of SYPRO Orange. The fluorophore is a styrene-like zwitterionic dye. It binds proteins selectively in the presence of nucleic acids, polysaccharides, and other molecules. It is also tolerant to SDS, and therefore can be used for SDS-PAGE gel staining. The dye interacts with the SDS coat around proteins in the gel, so it gives more consistent staining between different types of proteins compared to CBB staining, and never exhibits negative staining. It also stains glycoproteins well.
Staining with ProteOrange is about an order of magnitude more sensitive than Coumassie dye staining, and allows to detect about 3 ng of protein per band (30 ng is detection limit for Coumassie).
ProteOrange is supplied as a 5000x solution in DMSO. 1 mL of the solution is enough for the staining of around 200 minigels.
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Thiazole Orange NHS ester for labeling biomolecules (peptides, proteins, amino-DNA, etc.). Thiazole orange is a cyanine dye that is essentially dark in solution but has a bright fluorescence in double-stranded DNA and RNA.Linear Polyacrylamide for Nucleic Acid Precipitation
Nucleic acid coprecipitant. Suitable for PCR and RT-PCR reactions.General properties
| Appearance: | orange solution |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | 下載 |
| 產品規格 |
Spectral properties
| Excitation/absorption maximum, nm: | 470 |
| Emission maximum, nm: | 570 |
產品引用
- Somsen, B. A.; Sijbesma, E.; Leysen, S.; Honzejkova, K.; Visser, E. J.; Cossar, P. J.; Obšil, T.; Brunsveld, L.; Ottmann, C. Molecular Basis and Dual Ligand Regulation of Tetrameric Estrogen Receptor α/14-3-3ζ Protein Complex. Journal of Biological Chemistry, 2023, 299(7), 104855. doi: 10.1016/j.jbc.2023.104855
- Vasilev, R.; Gunitseva, N.; Shebanova, R.; Korzhenkov, A.; Vlaskina, A.; Evteeva, M.; Polushkina, I.; Nikitchina, N.; Toshchakov, S.; Kamenski, P.; Patrushev, M.; Mazunin, I. Targeted Modification of Mammalian DNA by a Novel Type V Cas12a Endonuclease from Ruminococcus bromii. International Journal of Molecular Sciences, 2022, 23(16), 9289. doi: 10.3390/ijms23169289
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