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Copper(II)-THPTA catalytic buffer, 1.5x

貨號	數量	價格	交貨時間
B5150	1 mL	$30	現貨
K5150	10 mL	$120	現貨
S5150	50 mL	$490	現貨

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Catalytic buffer is suitable for coupling of azide- or alkyne-modified protein with alkyne- or azide- bearing dye via Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC). THPTA ligand accelerates the reaction rate due to the stabilization of a catalytically active Cu (I). Moreover, the presence of water-soluble THPTA allows the protein labeling to be run in aqueous solution and, by chelating free copper, minimizes the generation of ROS (reactive oxygen species) and undesired damage of proteins. Aminoguanidine prevents connections of reactive aldehydes, which are products of dehydroascorbate hydrolysis, with arginine, N-terminal cysteine, and lysine side-chains. Ready-to-use 1.5× buffer provides all the necessary reagents to perform the CuAAC reaction, except a reagent for the reduction of Cu (II) into catalytically active Cu (I). As a reducing agent we recommend ascorbic acid.

Buffer composition: Cu (II), triethylammonium acetate buffer pH 6.8, THPTA ligand, aminoguanidine

建議協議

Conjugation of Alkyne- or Azide-modified Proteins with Dye Azides or Alkynes

計算機

解决方案计算器

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HPG (L-Homopropargylglycine)

Noncanonical amino acid analog of methionine with a terminal alkyne moiety for nascent protein synthesis detection.

ROX 疊氮化物，6-異構體 (ROX azide, 6-isomer)

ROX（羅丹明 X、羅丹明 101）染料的疊氮化物衍生物，用於通過點擊化學進行生物分子標記。純6-異構體。

AF 568 azide

Azide derivative of water-soluble AF 568 dye for labeling alkyne-containing biomolecules and fluorescent visualization of biological objects. Pure 6-isomer.

General properties

Storage conditions:	Storage: 12 months after receival at +4 or -20°C in the dark. Transportation: at room temperature for up to 3 weeks.
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	產品規格

This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.