Copper(II)-THPTA catalytic buffer, 1.5x
| 貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
|---|---|---|---|---|
| B5150 | 1 mL | $30 | 現貨 | |
| K5150 | 10 mL | $120 | 現貨 | |
| S5150 | 50 mL |
$490
|
現貨 |
Catalytic buffer is suitable for coupling of azide- or alkyne-modified protein with alkyne- or azide- bearing dye via Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC). THPTA ligand accelerates the reaction rate due to the stabilization of a catalytically active Cu (I). Moreover, the presence of water-soluble THPTA allows the protein labeling to be run in aqueous solution and, by chelating free copper, minimizes the generation of ROS (reactive oxygen species) and undesired damage of proteins. Aminoguanidine prevents connections of reactive aldehydes, which are products of dehydroascorbate hydrolysis, with arginine, N-terminal cysteine, and lysine side-chains. Ready-to-use 1.5x buffer provides all the necessary reagents to perform the CuAAC reaction, except a reagent for the reduction of Cu (II) into catalytically active Cu (I). As a reducing agent we recommend ascorbic acid.
Buffer composition: Cu (II), triethylammonium acetate buffer pH 6.8, THPTA ligand, aminoguanidine
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HPG (L-Homopropargylglycine)
Noncanonical amino acid analog of methionine with a terminal alkyne moiety for nascent protein synthesis detection.AF 568 NHS ester
AF 568 derivative for labeling proteins and other high- and low-molecular weight ligands with free amines.炔基馬來酰亞胺 (Alkyne maleimide)
馬來酰亞胺試劑用於將炔基官能團連接到蛋白質、多肽和其它含有 SH（硫醇）基團的分子上，包括半胱氨酸殘基。General properties
| Storage conditions: | Storage: 12 months after receival at +4 or -20°C in the dark. Transportation: at room temperature for up to 3 weeks. |
| MSDS: | 下載 |
| 產品規格 |
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