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Copper(II)-THPTA catalytic buffer, 1.5x

貨號 數量 價格 交貨時間
B5150 1 mL $30 現貨
K5150 10 mL $120 現貨
S5150 50 mL $490 現貨
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Catalytic buffer is suitable for coupling of azide- or alkyne-modified protein with alkyne- or azide- bearing dye via Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC). THPTA ligand accelerates the reaction rate due to the stabilization of a catalytically active Cu (I). Moreover, the presence of water-soluble THPTA allows the protein labeling to be run in aqueous solution and, by chelating free copper, minimizes the generation of ROS (reactive oxygen species) and undesired damage of proteins. Aminoguanidine prevents connections of reactive aldehydes, which are products of dehydroascorbate hydrolysis, with arginine, N-terminal cysteine, and lysine side-chains. Ready-to-use 1.5× buffer provides all the necessary reagents to perform the CuAAC reaction, except a reagent for the reduction of Cu (II) into catalytically active Cu (I). As a reducing agent we recommend ascorbic acid.

Buffer composition: Cu (II), triethylammonium acetate buffer pH 6.8, THPTA ligand, aminoguanidine

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THPTA配體

THPTA 是 Cu(I) 催化點擊化學的水溶性配體。該配體使銅穩定在 Cu(I) 氧化態。由於水溶性高,與該配體的反應不需要有機共溶劑。

General properties

Storage conditions: Storage: 12 months after receival at +4 or -20°C in the dark. Transportation: at room temperature for up to 3 weeks.
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