Perylene dU phosphoramidite
| 貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
|---|---|---|---|---|
| 14460 | 100 mg | $150 | 現貨 | |
| 24460 | 250 mg | $320 | 現貨 | |
| 44460 | 1 g |
$880
|
現貨 | |
| 64460 | 5 g | 請查詢 | 21 天 |
Perylene is a bright and extremely photostable fluorescent polycyclic aromatic hydrocarbon (PAH) label with a quantum yield approaching quantitative. Due to the low lifetime of fluorescence, this probe does not form excimers.
With this phosphoramidite, perylene can be introduced into DNA by automated oligonucleotide synthesis. Perylene is attached to the 5′ position of deoxyuridine (dU) through a triple bond and the fluorophore is electronically coupled to the deoxyuridine base. This coupling of dU and perylene makes the fluorescence sensitive to the base pairing of the dU portion of the molecule, allowing the discrimination between perfect and one base mismatched targets.
This amidite requires no special handling, coupling, or deprotection conditions. Recommended diluent is acetonitrile.
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General properties
| Appearance: | orange solid |
| Molecular weight: | 1005.1 |
| CAS number: | 908117-78-2 |
| Molecular formula: | C61H57N4O8P |
| Solubility: | good in dichloromethane and acetonitrile |
| Quality control: | NMR 1H, 31P, HPLC-MS (95%) |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | 下載 |
| 產品規格 |
Spectral properties
| Excitation/absorption maximum, nm: | 435; 408; 252 |
| ε, L⋅mol−1⋅cm−1: | 36000 |
| Emission maximum, nm: | 439; 467 |
| Fluorescence quantum yield: | 1.0 |
Oligo synthesis details
| Diluent: | acetonitrile |
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