此頁面上的某些內容不提供英文版本，將默認以當前可用的語言顯示。

MitoCLox, mitochondrial lipid peroxidation probe

Mitochondria Cell viability and apoptosis Microscopy Flow cytometry
貨號 數量 價格 交貨時間
3549-50ug 50 ug –   現貨
3549-500ug 500 ug $260 現貨
3549-1mg 1 mg $495 現貨
樣品請求
Found better price? Let us know and we will propose the way forward!

During the ferroptosis and mitochondrial stage of apoptosis, a mitochondria-specific phospholipid, cardiolipin (CL), undergoes peroxidation. MitoCLox is a mitochondria-targeted fluorescence probe that allows monitoring of this process in vivo.

MitoCLox consists of the BDP 581/591 fluorophore carrying a diene-containing moiety (C11) and linked with a triphenylphosphonium (TPP) residue via a long flexible linker with two amide bonds. MitoCLox is similar to MitoPerOx but has a longer linker and contains two (vs. one in MitoPerOx) peptide bonds. The flexible linker of MitoCLox mimics the SS-20 peptide (Phe-D-Arg-Phe-Lys-NH2), making the indicator specific for cardiolipin. The linker also increases the cellular permeability of MitoCLox due to additional positive charges.

The oxidation of the diene in MitoCLox results in a substantial increase in the fluorescence emission at 520 nm and a decrease in the initial fluorescence at 590 nm of the BDP 581/591 fluorophore. Thus, the oxidation of MitoCLox could be measured either as a decrease of absorbance at 588 nm or as an increase of fluorescence emission in the ratiometric mode at 520/590 nm [1].

MitoCLox is accumulated in the mitochondria of living cells. Maximal accumulation of MitoCLox in the cells is reached in 45-60 min. After removing MitoCLox from the medium, the fluorescence of the cells slowly decreased and reached 50% of the maximum in approximately 1 h. The recommended working concentration of MitoCLox is 100-200 nM [2].


[1] Lyamzaev K.G. et al. MitoCLox: A Novel Mitochondria-Targeted Fluorescent Probe for Tracing Lipid Peroxidation. Oxid. Med. Cell Longev. 2019:9710208.

[2] Lyamzaev K.G. et al. Novel Fluorescent Mitochondria-Targeted Probe MitoCLox Reports Lipid Peroxidation in Response to Oxidative Stress In Vivo. Oxid. Med. Cell Longev. 2020:3631272.
以
ChemDraw 格式下載

顧客還購買了這個產品

BTTAA配體 (BTTAA ligand)

BTTAA 是銅 (I) 催化的疊氮化物-炔環加成 (CuAAC) 的最新一代水溶性配體，可顯著加快反應速率並抑制細胞毒性。

FAM NHS 酯，6-異構體 (FAM NHS ester, 6-isomer)

活化的螢光素 N-羥基琥珀醯亞胺酯 (FAM)，純 6-異構體。

Cyanine 5.5胺 (Cyanine5.5 amine)

Cyanine5.5 的氨基衍生物，一種遠紅光發射的螢光花菁染料。
將此產品添加到購物車並
立即在線獲取免費快遞服務

General properties

Appearance: black powder
Molecular weight: 901.69
Molecular formula: C50H53BBrF2N4O2P
Solubility: good in DMSO
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
MSDS: 下載
產品規格

Spectral properties

Excitation/absorption maximum, nm: 585
ε, L⋅mol−1⋅cm−1: 138500
Emission maximum, nm: 595
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
已添加您的物品. 查看您的 購物車 或 繼續結賬
物品數量不正確.