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LUTOX® Green Ready Stain for Flow Cytometry

貨號 數量 價格 交貨時間
1A410
/ 50 assays
2 mL $104 20 天
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LUTOX® Green is a green-fluorescent nucleic acid stain used as a nuclear and chromosome counterstain. The dye is impermeant to live cells with compromised plasma membranes, making it a good indicator of dead cells. LUTOX Green exhibits more than 500-fold fluorescence enhancement upon binding nucleic acids with an emission peak of 523 nm. The high brightness of the signal allows the use of LUTOX Green to determine the viability of both eukaryotic and bacterial (Gram-positive and Gram-negative) cells by flow cytometers, fluorometers, fluorescence microscopes, or microplate readers.

We also offer LUTOX Green as a 5 mM solution in DMSO.

Protocol

Step 1: Add 2 drops per 106 cells in 1 mL.

Step 2: Incubate for 15 minutes at 25 °C.

Step 3: Proceed with flow cytometry.

General properties

Appearance: colorless solution
Quality control: NMR 1H and HPLC-MS (95+%), functional testing
Storage conditions: 12 months after receival at 2-8°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate.
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