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LUCS® 5, far-red fluorescent nucleic acid stain

Nuclei Cell viability and apoptosis Microscopy Flow cytometry
貨號 數量 價格 交貨時間
1L010 50 uL, 5 mM/water $169 現貨
2L010 250 uL, 5 mM/water $799 現貨
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LUCS® 5 (1,5-bis{[2-(di-methylamino) ethyl]amino}-4,8-dihydroxyanthracene-9,10-dione, DRAQ5®) is a cell-permeant far-red fluorescent DNA dye for live and fixed cell imaging and analysis.

LUCS® 5 readily permeates the cell membranes and intercalates between A-T bases of double-strained DNA (dsDNA). The dye has a high affinity to dsDNA and shows negligible binding to RNA and mtDNA. LUCS® 5 does not exhibit a fluorescence enhancement on DNA binding; thus, the measured fluorescence is proportional and stoichiometric to the quantity of nuclear DNA content of the cell.

Due to its cell permeability, LUCS® 5 is useful for assessing DNA content and cell cycle but is not suitable for use as a viability dye. As with other cell­-permeant DNA intercalating dyes, LUCS® 5 may inhibit cell division in long-term assays, so this effect should be tested before the experiment.

In microscopy and high-content screening, LUCS® 5 is useful as a nuclear counterstain for both live and fixed specimens. In flow cytometry, LUCS® 5 allows direct blood and bone marrow cell discrimination without preliminary red blood cell lysis, fixation, permeabilization, or RNase treatment.

LUCS® 5 has absorbance peaks at 617 nm and 665 nm and an emission peak at 701 nm (when intercalated into dsDNA). Thus, this dye is spectrally compatible with the most common labels, such as GFP, FITC, R-PE, or RFP. LUCS® 5 also has high photostability and demonstrates no photobleaching effect during imaging.

LUCS® 5 solution should be stored at 2-8 °C. Do NOT freeze the solution! LUCS® 5 may precipitate out of the solution when frozen. Re-dissolving it may not be easy.

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General properties

Appearance: blue-violet solution
Molecular weight: 485.41
CAS number: 252903-95-0
Molecular formula: C22H30Cl2N4O4
IUPAC name: 1,5-bis{[2-(di-methylamino) ethyl]amino}-4, 8-dihydroxyanthracene-9,10-dione
Solubility: water, DMSO
Quality control: NMR 1H and HPLC-MS (95+%)
Storage conditions: Store at temperature between +2 °C and +8 °C. Do not freeze! Transportation: at room temperature for up to 3 weeks.
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產品規格

Spectral properties

Excitation/absorption maximum, nm: 665 (complex)
Emission maximum, nm: 701 (complex)
DRAQ5™ is a registered trademark of BioStatus Ltd.
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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