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JOE phosphoramidite, 5-isomer
貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
---|---|---|---|---|
C1160 | 100 mg | – | 現貨 | |
D1160 | 250 mg | $165 | 現貨 | |
F1160 | 1 g | $575 | 現貨 | |
G1160 | 5 g | $2230 | 現貨 | |
H1160 | 10 g | $3300 | 現貨 |
JOE is a xanthene dye, fluorescein derivative possessing two chloro- and two methoxy substituents. This fluorophore is a useful label for oligonucleotides. Its absorption and emission spectra are located between FAM and TAMRA.
The fluorophore can be introduced into oligonucleotide using this phosphoramidite. It tolerates standard ammonium deblock conditions. This product contains a pure isomer of 5-JOE dye.
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磺基-Cyanine5 NHS 酯 (sulfo-Cyanine5 NHS ester)
水溶性磺基-Cyanine5 琥珀酰亞胺酯,用於在水相中標記各種含胺分子。dsGreen 用於實時定量PCR, 100× (dsGreen for Real-Time PCR, 100×)
用於實時定量PCR 的 dsGreen 儲備液,100×sulfo-Cyanine5.5 azide
sulfo-Cyanine5.5 azide is a derivative of water-soluble far-red emitting fluorescent cyanine dye. The azide group can be conjugated by either copper-catalyzed or copper-free click chemistry with alkynes and cycloalkynes.General properties
Appearance: | colorless solid |
Molecular weight: | 972.88 |
Molecular formula: | C48H60N3Cl2O12P |
Solubility: | good in DCM, acetonitrile |
Quality control: | NMR 1H, 31P, HPLC-MS (95%), functional testing |
Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
MSDS: | 下載 |
產品規格 |
Spectral properties
Excitation/absorption maximum, nm: | 533 |
ε, L⋅mol−1⋅cm−1: | 75000 |
Emission maximum, nm: | 554 |
Fluorescence quantum yield: | 0.61 |
CF260: | 0.36 |
CF280: | 0.28 |
Oligo synthesis details
Diluent: | acetonitrile |
Coupling conditions: | standard coupling, identical to normal nucleobases |
Cleavage conditions: | ammonia, 2 h at room temperature |
Deprotection conditions: | identical to protected nucleobases |
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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