DusQ 2 CPG 1000
| 貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
|---|---|---|---|---|
| 15960 | 100 mg | – | 現貨 | |
| 35960 | 500 mg |
$530
|
現貨 | |
| 45960 | 1 g |
$990
|
現貨 | |
| 55960 | 5 g | 請查詢 | 現貨 |
This modified support is intended for the synthesis of oligonucleotides with DusQ 2 quencher at the 3’ end. Pore size of 1000 Å is recommended for the synthesis of oligonucleotides of up to 120 bases in length.
DusQ 2 is a fluorescence quencher with absorption within the range of 560 to 670 nm. It is ideal for effective FRET quenching of fluorophores with emission in this range. The quencher is also used in hybridization probes with static and combined quenching. Its quenching effectiveness does not depend on overlapping of fluorophore and quencher spectra, thus allowing for effective quenching of the broad spectrum of fluorophores, including those with emission in the red and far-red part of the spectrum. Thus, DusQ 2 can be used with such fluorophores (including but not limited to) as Cyanine3, TAMRA, ROX, Cyanine3.5, Cyanine5, Cyanine5.5.
Usage
Coupling: Standard conditions identical to normal nucleobases.
Deprotection: 2 hours at room temperature using concentrated ammonia or 10 min at 65 °C using AMA mixture, concentrated aqueous ammonia/40% methylamine (1:1). Deprotection conditions depend on oligonucleotide composition and nucleobase protecting groups, as well as additional modifications, if present.
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Cyanine5.5 phosphoramidite
Amidite for the synthesis of oligonucleotides and qPCR probes bearing 5'-terminal dye Cyanine5.5. Useful dye for multiplex qPCR.
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六角疊氮化物, 6-異構體 (HEX azide, 6-isomer)
綠色-黃色發射 HEX 染料的疊氮化物，通過 [3+2]-偶極環加成與寡核苷酸偶合。DusQ 2 phosphoramidite
DusQ 2 phosphoramidite for the synthesis of oligonucleotides labeled with DusQ 2 quencher at 3'-end, 5'-end and internal position. The phosphoramidite has DMT protection and can be used for cartridge purification.
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General properties
| Appearance: | dark blue beads |
| Quality control: | NMR 1H and HPLC-MS (95%) of bound reagent, loading measurement, functional testing in oligo synthesis. |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | 下載 |
| 產品規格 |
Spectral properties
| Excitation/absorption maximum, nm: | 552 |
| CF260: | 0.31 |
| CF280: | 0.26 |
Oligo synthesis details
| Pore size, Å: | 1000 |
| Typical loading, umol/g: | 30−50 |
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