DusQ 1 CPG 1000
| 貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
|---|---|---|---|---|
| 14960 | 100 mg | – | 現貨 | |
| 34960 | 500 mg |
$530
|
現貨 | |
| 44960 | 1 g |
$990
|
現貨 | |
| 54960 | 5 g | 請查詢 | 現貨 |
This support with a pore size of 1000 Å is intended for the automated synthesis of oligonucleotides of up to 120 bases in length modified with DusQ1 dark quencher at the 3’ end.
Non-fluorescent DusQ1 quencher exhibits the strongest absorption within the range of 480 to 580 nm; its absorption maximum is at 534 nm. It is suitable for quenching (a combination of static and dynamic quenching) of many fluorophores, including Biosearch Blue™, Marina Blue™, Edans, Bothell Blue, FAM™, JOE™, VIC™, R6G, HEX™, TET™ and Yakima Yellow™. It can be used for the synthesis of hybridization probes such as TaqMan, Molecular Beacon, Scorpion.
Usage
Coupling: Standard conditions identical to normal nucleobases.
Deprotection: 2 hours at room temperature using concentrated ammonia or 10 min at 65 °C using AMA mixture, concentrated aqueous ammonia/40% methylamine (1:1). Deprotection conditions depend on oligonucleotide composition and nucleobase protecting groups, as well as additional modifications, if present.
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DusQ 1 amidite, 5'-terminal
Terminal phosphoramidite for labeling oligonucleotides at the 5' end with DusQ 1 quencher.
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Inosine (dI) phosphoramidite
Dimethoxytrityl deoxyinosine (Dmt-dI) phosphoramidite for the synthesis of modified DNA oligonucleotides.通用 CPG II 型，1000A (Universal CPG type II, 1000A)
這種通用支持物旨在用於寡核苷酸的自動合成，以加速解封閉過程中的去磷酸化過程。對於多達 120 個堿基的寡核苷酸合成，建議使用 1000 Å 的孔徑。General properties
| Appearance: | red beads |
| Quality control: | NMR 1H and HPLC-MS (95%) of bound reagent, loading measurement, functional testing in oligo synthesis. |
| Storage conditions: | Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate. |
| MSDS: | 下載 |
| 產品規格 |
Spectral properties
| Excitation/absorption maximum, nm: | 522 |
| ε, L⋅mol−1⋅cm−1: | 27300 |
| CF260: | 0.17 |
| CF280: | 0.10 |
Oligo synthesis details
| Pore size, Å: | 1000 |
| Typical loading, umol/g: | 30−50 |
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