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DusQ 1 CPG 1000

貨號 數量 價格 交貨時間
14960 100 mg –   現貨
34960 500 mg $530 現貨
44960 1 g $990 現貨
54960 5 g 請查詢 現貨
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This support with a pore size of 1000 Å is intended for the automated synthesis of oligonucleotides of up to 120 bases in length modified with DusQ1 dark quencher at the 3’ end.

Non-fluorescent DusQ1 quencher exhibits the strongest absorption within the range of 480 to 580 nm; its absorption maximum is at 534 nm. It is suitable for quenching (a combination of static and dynamic quenching) of many fluorophores, including Biosearch Blue™, Marina Blue™, Edans, Bothell Blue, FAM™, JOE™, VIC™, R6G, HEX™, TET™ and Yakima Yellow™. It can be used for the synthesis of hybridization probes such as TaqMan, Molecular Beacon, Scorpion.

Usage

Coupling: Standard conditions identical to normal nucleobases.

Deprotection: 2 hours at room temperature using concentrated ammonia or 10 min at 65 °C using AMA mixture, concentrated aqueous ammonia/40% methylamine (1:1). Deprotection conditions depend on oligonucleotide composition and nucleobase protecting groups, as well as additional modifications, if present.

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Phosphate CPG 1000 solid support

Phosphate modifier CPG for the solid phase synthesis of long (up to 120mer) oligonucleotides containing 3 prime phosphate modification.

DusQ 2 CPG 500

Controlled pore glass (CPG) 500 modified support for the synthesis of oligonucleotides with DusQ 2 quencher at the 3’ end. Compatible with standard deblocking conditions.
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R6G phosphoramidite, 6-isomer

Phosphoramidite for synthesis of oligonucleotides labeled at the 5'-terminus with rhodamine 6G (R6G). Pure 6-isomer.
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General properties

Appearance: red beads
Quality control: NMR 1H and HPLC-MS (95%) of bound reagent, loading measurement, functional testing in oligo synthesis.
Storage conditions: Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS: 下載
產品規格

Spectral properties

Excitation/absorption maximum, nm: 522
ε, L⋅mol−1⋅cm−1: 27300
CF260: 0.17
CF280: 0.10

Oligo synthesis details

Pore size, Å: 1000
Typical loading, umol/g: 30−50
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