DAPI, blue fluorescent nucleic acid stain
| 貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
|---|---|---|---|---|
| 2996-1mL | 1 mL, 1 mg/mL in Water | $75 | 現貨 | |
| 2996-50uL | 50 uL, 1 mg/mL in Water | – | 現貨 | |
| 2996-1mg | 1 mg | $30 | 現貨 | |
| 2996-10mg | 10 mg | $110 | 現貨 |
DAPI (4′,6-diamidino-2-phenylindole) is a blue-emitting fluorescent dye that binds strongly to adenine-thymine-rich regions in DNA. It is used extensively in fluorescence microscopy, flow cytometry for chromosome staining and as a nuclear counterstain. DAPI is generally used to stain fixed cells since the dye is membrane impermeant, although the dye can enter live cells when used at high concentrations.
When bound to double-stranded DNA, DAPI exhibits ~20-fold enhancement of fluorescence with an absorption maximum at 355 nm and emission maximum at 460 nm. DAPI also binds to RNA, though it is not as strongly fluorescent. When bound to RNA, its emission spectrum shifts to around 500 nm.
There is a slight fluorescence overlap between DAPI and green-fluorescent molecules like fluorescein and GFP. Use spectral unmixing if extremely precise image analysis is required.
DAPI is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting DNA and detecting annealed probes in blotting applications by specifically visualizing the double-stranded complex. Also, DAPI staining is a sensitive and specific detection method for mycoplasma.
The working concentration of DAPI for staining eukaryotic cells is usually 1 μg/mL. Still, it depends on the cell type and density, so the exact dilution of the dye should be determined experimentally.
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YO-TAP-1，綠色螢光核酸染料 (YO-TAP-1, green fluorescent nucleic acid stain)
YO-TAP-1 是一種細胞不可滲透的綠色螢光核酸染料。 YO-TAP-1 的化學性質與 YO-PRO®-1 相同。磺基-Cyanine5.5胺 (sulfo-Cyanine5.5 amine)
sulfo-Cyanine5.5 是一種遠紅/近紅外螢光團，具有高親水性和水溶性。這是一種對親電試劑具有反應活性的胺衍生物。它還可用於酶促轉氨標記。銅 (II)-TВTA 催化緩衝液，1.5x (Copper(II)-TВTA catalytic buffer, 1.5x)
即用型催化緩衝液含有銅(II) 和 TBTA 配體。適用於核酸和小分子的點擊化學修飾。General properties
| Appearance: | yellow solid / solution |
| Molecular weight: | 350.25 |
| CAS number: | 28718-90-3 |
| Molecular formula: | C16H17Cl2N5 |
| Solubility: | good in DMF, DMSO |
| Quality control: | NMR 1H and HPLC-MS (95+%) |
| Storage conditions: | 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Desiccate. |
| MSDS: | 下載 |
| 產品規格 |
Spectral properties
| Excitation/absorption maximum, nm: | 355 |
| ε, L⋅mol−1⋅cm−1: | 28800 |
| Emission maximum, nm: | 460 |
產品引用
- Samatoshenkov, I.V.; Aimaletdinov, A.M.; Zakirova, E.Y.; Chelyshev, Y.A.; Samatoshenkova, J.M.; Kadyrov, M.S.; Kniazev, E.A.; Salakhov, B.I.; Mukhamedshina, Y.O. The Combined Delivery of the Vegf, Ang, and Gdnf Genes Stimulates Angiogenesis and Improves Post-Ischemic Innervation and Regeneration in Skeletal Muscle. Current Issues in Molecular Biology, 2024, 46(8), 8611-8626. doi: 10.3390/cimb46080507
- Potapenko, E.Y..; Kashko, N.D.; Knorre, D.A. Spontaneous Mutations in Saccharomyces cerevisiae mtDNA Increase Cell-to-Cell Variation in mtDNA Amount. International Journal of Molecular Sciences, 2023, 24(24), 17413. doi: 10.3390/ijms242417413
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