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Cyanine3 tetrazine

花青3 四嗪類 Copper-free click chemistry

貨號	數量	價格	交貨時間
110E0	1 mg	$125	現貨
210E0	5 mg	$260	現貨
410E0	25 mg	$510	現貨
510E0	50 mg	$895	現貨
610E0	100 mg	$1490	現貨

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Cyanine3 tetrazine is a fluorescent dye with tetrazine moiety for a conjugation reaction called TCO (trans-cyclooctene) ligation. Actually, tetrazines react not only with trans-cyclooctenes, but also with cyclopropenes and strained non-benzo-fused cycloalkynes.

Cyanine3 is a moderately hydrophobic dye, enabling labeling in non-aqueous environment as well as in aqueous media containing some organic co-solvent like acetonitrile or DMSO. For reactions in purely aqueous media, a sulfonated version of this reagent is available.

以
ChemDraw 格式下載

General properties

Appearance:	dark red solid
Mass spec M+ increment:	611.4
Molecular weight:	727.64
CAS number:	2183473-57-4
Molecular formula:	C₄₀H₄₆N₇BF₄O
Solubility:	good in DCM, DMSO, DMF
Quality control:	NMR ¹H, HPLC-MS (95%)
Storage conditions:	Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
MSDS:	下載
	產品規格

Spectral properties

Excitation/absorption maximum, nm:	555
ε, L⋅mol⁻¹⋅cm⁻¹:	150000
Emission maximum, nm:	570
Fluorescence quantum yield:	0.31
CF₂₆₀:	0.04
CF₂₈₀:	0.09

產品引用

Bai, M.; Cao, X.; Huang, W.; Chen, F.; Zhao, Y.; Xue, J.; Li, Y.; Cheng, Y.; Zhang, L.; Zhao, Y. Visualizing Newly Synthesized RNA by Bioorthogonal Labeling-primed DNA Amplification. Analytical Chemistry, 2020, 92(12), 8444–8449. doi: 10.1021/acs.analchem.0c01161
Moutsiopoulou, A.; Broyles, D.; Joda, H.; Dikici, E.; Kaur, A.; Kaifer, A.E.; Daunert, S.; Deo, S.K. Bioluminescent Protein-Inhibitor Pair in the Design of a Molecular Aptamer Beacon Biosensing System. Analytical Chemistry, 2020, 92(11), 7393–7398. doi: 10.1021/acs.analchem.0c00518
Chen, F.; Bai, M.; Cao, X.; Zhao, Y.; Xue, J.; Zhao, Y. Click-encoded rolling FISH for visualizing single-cell RNA polyadenylation and structures. Nucleic Acids Research, 2019, 47(22), e145. doi: 10.1093/nar/gkz852