Biotin-11-dUTP
貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
---|---|---|---|---|
1553-250nmol | 250 nmol | $140 | 20 天 | |
1553-500nmol | 500 nmol | $235 | 現貨 | |
1553-1umol | 1 umol | $360 | 現貨 | |
1553-5umol | 5 umol | 請查詢 | 現貨 |
Biotinylated deoxyuridine triphosphate (dUTP) can be used for DNA labeling using various methods, including Nick-translation and 3’-terminal non-radioactive labeling. Generated probes can be used in various hybridization experiments such as Southern blot, Northern blot, dot blot, or FISH. Biotin-labeled probes are detected with horseradish peroxidase-conjugated streptavidin or other biomolecule conjugates binding biotin.
«11» in the compound name indicates the length of the linker between dUTP and biotin; this linker increases the efficiency of labeled dUTP incorporation into DNA and interaction of biotin with specific proteins such as avidin/streptavidin or anti-biotin antibodies.
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Biotin-11-dCTP
Biotinylated dCTP with 11-atom linker for non-radioactive DNA-labeling.sulfo-Cyanine3 dUTP
Fluorescent dNTP for the labeling of DNA with sulfo-Cyanine3.PreciseGreen dsDNA 定量試劑,200× (PreciseGreen dsDNA Quantification Reagent, 200×)
PreciseGreen 是一種染料,可用於在RNA、ssDNA 和其他雜質存在的情況下對dsDNA進行精確熒光定量。該染料在熒光素通道中表現出明亮的熒光,在pg/mLng/mL 範圍內對 DNA 濃度提供線性響應。General properties
Appearance: | colorless solid |
Molecular weight: | 878.54 |
Molecular formula: | C28H40N6Li3O17P3S |
IUPAC name: | ((2R,3S,5R)-5-(2,4-dioxo-5-(3-(6-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanamido)prop-1-yn-1-yl)-3,4-dihydropyrimidin-1(2H)-yl)-3-hydroxytetrahydrofuran-2-yl)methyl hydrogen triphosphate |
Solubility: | soluble in water |
Quality control: | HPLC-MS (95%), testing in enzymatic reaction |
Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid excessive freeze-thaw cycles. |
MSDS: | 下載 |
產品規格 |
產品引用
- Krasikova, A.; Kulikova, T.; Schelkunov, M.; Makarova, N.; Fedotova, A.; Berngardt, V.; Maslova, A.; Fedorov, A. The first chicken oocyte nucleus whole transcriptomic profile defines the spectrum of maternal mRNA and non-coding RNA genes transcribed by the lampbrush chromosomes. bioRxiv, 2024, preprint. doi: 10.1101/2024.02.05.577752
- Krasikova, A.; Kulikova, T.; Rodriguez Ramos, J. S.; Maslova, A. Assignment of the Somatic A/B Compartments to Chromatin Domains in Giant Transcriptionally Active Lampbrush Chromosomes. Epigenetics & Chromatin, 2023, 16(1), 24. doi: 10.1186/s13072-023-00499-2