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Biotin-11-dUTP
| 貨號 | 數量 | 價格 | 交貨時間 | 購買此產品 |
|---|---|---|---|---|
| 1553-250nmol | 250 nmol | $140 | 20 天 | |
| 1553-500nmol | 500 nmol | $235 | 現貨 | |
| 1553-1umol | 1 umol | $360 | 現貨 | |
| 1553-5umol | 5 umol | 請查詢 | 現貨 |
Biotinylated deoxyuridine triphosphate (dUTP) can be used for DNA labeling using various methods, including Nick-translation and 3’-terminal non-radioactive labeling. Generated probes can be used in various hybridization experiments such as Southern blot, Northern blot, dot blot, or FISH. Biotin-labeled probes are detected with horseradish peroxidase-conjugated streptavidin or other biomolecule conjugates binding biotin.
«11» in the compound name indicates the length of the linker between dUTP and biotin; this linker increases the efficiency of labeled dUTP incorporation into DNA and interaction of biotin with specific proteins such as avidin/streptavidin or anti-biotin antibodies.
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用於 qPCR 的 ROX 參考染料 (ROX reference dye for qPCR)
ROX 是一種用於 qPCR 擴增的被動參比染料。該染料可用於均一化 qPCR 中報告染料的螢光強度。DMSO (dimethyl sulfoxide), labeling grade
Pure dimethyl sulfoxide (DMSO) solvent with quality optimized for bioconjugation reactions.FAM-11-dUTP, 6-isomer
FAM-derivative of dUTP with green emission for DNA labeling.General properties
| Appearance: | colorless solid |
| Molecular weight: | 878.54 |
| Molecular formula: | C28H40N6Li3O17P3S |
| IUPAC name: | ((2R,3S,5R)-5-(2,4-dioxo-5-(3-(6-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanamido)prop-1-yn-1-yl)-3,4-dihydropyrimidin-1(2H)-yl)-3-hydroxytetrahydrofuran-2-yl)methyl hydrogen triphosphate |
| Solubility: | soluble in water |
| Quality control: | HPLC-MS (95%), testing in enzymatic reaction |
| Storage conditions: | Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid excessive freeze-thaw cycles. |
| MSDS: | 下載 |
| 產品規格 |
產品引用
- Krasikova, A.; Kulikova, T.; Schelkunov, M.; Makarova, N.; Fedotova, A.; Berngardt, V.; Maslova, A.; Fedorov, A. The first chicken oocyte nucleus whole transcriptomic profile defines the spectrum of maternal mRNA and non-coding RNA genes transcribed by the lampbrush chromosomes. bioRxiv, 2024, preprint. doi: 10.1101/2024.02.05.577752
- Krasikova, A.; Kulikova, T.; Rodriguez Ramos, J. S.; Maslova, A. Assignment of the Somatic A/B Compartments to Chromatin Domains in Giant Transcriptionally Active Lampbrush Chromosomes. Epigenetics & Chromatin, 2023, 16(1), 24. doi: 10.1186/s13072-023-00499-2
This Product is offered and sold for research purposes only. It has not been tested for safety and efficacy in food, drug, medical device, cosmetic, commercial or any other use. Supply does not express or imply authorization to use for any other purpose, including, without limitation, in vitro diagnostic purposes, in the manufacture of food or pharmaceutical products, in medical devices or in cosmetic products.
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