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Biotin-11-dCTP

Triphosphates
貨號 數量 價格 交貨時間
2715-250nmol 250 nmol $140 現貨
2715-500nmol 500 nmol $235 現貨
2715-1umol 1 umol $360 現貨
2715-5umol 5 umol 請查詢 現貨
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Biotin-11-dCTP is a deoxycytidine triphosphate (dCTP) linked to a biotine molecule via an 11-atom linker, which allows efficient interaction with avidin and streptavidin; incorporation into DNA proceeds as with natural dCTP.

Biotin-11-dCTP is a substrate for Klenow fragment, T4 and Taq DNA-polymerases, reverse transcriptase, and terminal transferase. Biotinylated DNA can be detected using alkaline phosphatase-conjugated avidin/streptavidin or relevant fluorescent-labeled antibodies.

Biotin-labeled DNA/cDNA probes can be used for fluorescent hybridization in situ or microarray gene profiling.

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EdU（5-乙炔基-2'-脫氧尿苷）

EdU 是一種核苷，通過細胞內的酶摻入到復製的 DNA 中。之後，可以通過與熒光染料疊氮化物的點擊化學反應來顯色細胞中含有的DNA，以揭示細胞增殖情況。

General properties

Appearance: colorless solid
Molecular weight: 877.48
Molecular formula: C28H41Li3N7O16P3S
IUPAC name: ((2R,3S,5R)-5-(4-amino-2-oxo-5-(3-(6-(5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanamido)prop-1-yn-1-yl)-3,4-dihydropyrimidin-1(2H)-yl)-3-hydroxytetrahydrofuran-2-yl)methyl hydrogen triphosphate
Solubility: soluble in water
Quality control: HPLC-MS (95%), testing in enzymatic reaction
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid excessive freeze-thaw cycles.
MSDS: 下載
產品規格

產品引用

  1. Feng, X.; Cui, X.; Zhang, L.-S.; Ye, C.; Wang, P.; Zhong, Y.; Wu, T.; Zheng, Z.; He, C. Sequencing of N6-Methyl-Deoxyadenosine at Single-Base Resolution across the Mammalian Genome. Molecular Cell, 2024, 84(3), 596-610.e6. doi: 10.1016/j.molcel.2023.12.021
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