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AF 488 carboxylic acid

貨號 數量 價格 交貨時間
11890 1 mg $125 現貨
21890 5 mg $325 現貨
41890 25 mg $950 現貨
51890 50 mg $1625 現貨
61890 100 mg $2090 現貨
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AF 488 is a bright green-fluorescent dye that is commonly used in microscopy and cell assays because of its photostability. AF 488 can be used with DAPI and is well suited to multiplex assay. AF 488 has high quantum yield and stable fluorescence within the pH range from 4 to 10.

AF 488 carboxylic acid is a non-reactive AF 488 form that can be used as a reference standard in experiments where AF 488 conjugates are used. The carboxylic acid can be also used for the synthesis of activated esters [such as sulfo-NHS, TFP (2,3,5,6-tetrafluorophenol) and STP (4-sulfo-2,3,5,6-tetrafluorophenol)] or modified with hydrazines, hydroxylamines, or amines in aqueous solutions using water-soluble carbodiimides. Thus, this derivative can be conjugated to molecules that contain amino groups, such as proteins, antibodies, and peptides. Therefore, AF 488 carboxylic acid is used during solid-phase peptide synthesis for peptide modification in situ in the presence of activating agents such as HATU.

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General properties

Appearance: orange crystals
Molecular weight: 648.75
Molecular formula: C21H11K3N2O11S2
IUPAC name: 4-(6-amino-3-iminio-4,5-disulfonato-3H-xanthen-9-yl)isophthalate
Solubility: good in DMSO, DMF
Quality control: NMR 1H, HPLC-MS (95%)
Storage conditions: Storage: 12 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light.
MSDS: 下載
產品規格

Spectral properties

Excitation/absorption maximum, nm: 495
ε, L⋅mol−1⋅cm−1: 71800
Emission maximum, nm: 519
Fluorescence quantum yield: 0.91
CF260: 0.16
CF280: 0.10
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