ProbeMaster® Lyo: universal line of lyophilized mixes for PCR and real-time PCR from Lumiprobe
The ProbeMaster® Lyo series from Lumiprobe is a line of ready-to-use lyophilized mixes for PCR and real-time PCR. We created them to make DNA amplification as convenient as possible: the mixes do not require freezing, store well at room temperature, and allow you to quickly start the reaction by simply adding water, oligonucleotides, and the sample.
Key features
- Easy to use — Ready-made mix reduces reaction setup time and lowers the risk of contamination.
- Convenient storage — Reconstituted mix is stable for 30 days when stored at 4 °C and withstands up to 5 freeze/thaw cycles.
- Optimized lyophilization — Each lyophilized pellet, after dissolution, is sufficient for 100 reactions of 25 µL volume.
- Hot‑start technology — Use of antibody‑dependent HS Taq polymerase eliminates nonspecific reactions and primer‑dimer formation, ensuring high purity and specificity.
- Additional activity — The polymerase possesses 5’→3′ exonuclease and terminal transferase activity, enabling real‑time PCR with TaqMan probes as well as TA cloning of PCR products.
Overview of mixes in the series
For real‑time PCR (qPCR)
| Name and composition | Application |
|---|---|
| ProbeMaster® Lyo UNI — universal basic mix containing polymerase, buffer, and dNTP | Any qPCR — with your intercalating dye or hydrolyzable probes (TaqMan) |
| ProbeMaster® Lyo ROX — mix with ROX dye for signal normalization | High‑precision qPCR for quantitative analysis, gene expression, and genotyping (on instruments requiring a passive ROX reference) |
| ProbeMaster® Lyo dsGreen — master mix containing the intercalating dye dsGreen | Probe-free qPCR — detection of reaction products via dsGreen dye fluorescence in the FAM channel |
| ProbeMaster® Lyo UDG — mix with UDG/dUTP system for control of amplicon contamination | qPCR with contamination control to prevent false‑positive results due to carryover from previous reactions |
For standard PCR with gel electrophoresis
| Name and composition | Application |
|---|---|
| ProbeMaster® Lyo GEL — mix containing loading dyes (bromophenol blue, xylene cyanol) | PCR with direct gel loading — after the reaction, the product can be loaded directly onto an agarose gel without adding loading buffer |
| ProbeMaster® Lyo GEL UDG — mix for PCR with gel electrophoresis and UDG/dUTP system | Standard PCR with contamination control when false‑positive results must be prevented, especially when using gel electrophoresis and frequently amplifying the same fragments |
Just add water, oligonucleotides, and the sample, then start the reaction!
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