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• PKH800 Cell Membrane Labeling Kit

PKH800 Cell Membrane Labeling Kit

The ready-to-use kit contains all necessary reagents to label cell membranes with PKH800 dye for evaluating cell migration or proliferation.

The kit contains PKH800 cyanine dye with lipophilic groups, which allow rapid and non-covalent incorporation into cell membranes of almost any cells without influence on cell receptors or transmembrane proteins. The cells preserve their biological properties and ability to proliferate, which makes PKH800 an excellent fluorophore for studying plant and animal cells in vivo and in vitro. It can also be used for studying membrane vesicles.

PKH800 is a near-infrared fluorescent dye with an emission maximum at 797 nm. It can be used with other dyes in multicolor assays and, especially, in applications requiring low sample background fluorescence. PKH800 is rather stable, and PKH800-labeled cells can be studied by microscopy or flow cytometry within several days after staining.

The number of measurements using the kit can vary depending on the cell count in the sample:

• The kit containing 100 µL of PHK800 allows analyzing 25 samples of 20 million cells each, using the final PKH800 concentration of 2×10^-6 M.
• The kit containing 500 µL of PHK800 allows analyzing 125 samples of 20 million cells each, using the final PKH800 concentration of 2×10^-6 M.

We recommend choosing the necessary cell count and concentration depending on the cell type and study objectives.