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• Mitochondrial Membrane Potential Apoptosis Kit, with LumiTracker® Mito Red CMXRos & Annexin V-AF 488

Mitochondrial Membrane Potential Apoptosis Kit, with LumiTracker® Mito Red CMXRos & Annexin V-AF 488

This kit is designed for a fast and convenient assay of two key indicators of apoptosis: the externalization of phosphatidylserine (Annexin V-AF 488) and alterations in mitochondrial membrane potential (LumiTracker^® Mito Red CMXRos).

Annexin V (or Annexin A5) belongs to the phospholipid-binding Annexin family of intracellular proteins. It is commonly used to detect apoptotic cells by its ability to specifically bind to phosphatidylserine, which migrates from the inner to the outer leaflet of the plasma membrane during the early stages of apoptosis. This kit contains recombinant Annexin V conjugated with AF 488, a bright, photostable green fluorophore with spectral characteristics similar to FITC.

LumiTracker^® Mito Red CMXRos is a cationic, red-fluorescent dye that passively diffuses across the plasma membrane and selectively accumulates in active mitochondria based on their membrane potential. Healthy cells have a high mitochondrial membrane potential, and its decrease is considered a marker of the early stage of apoptosis.

After staining a population of cells with Annexin V-AF 488 and LumiTracker^® Mito Red CMXRos dye in the supplied binding buffer, live cells will show minimal green and intense red fluorescence. In contrast, apoptotic cells will display green fluorescence and reduced red fluorescence. These different cell populations can be easily distinguished using a flow cytometer, with both dyes being excited by the 488 nm line of an argon-ion laser.