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QuDye dsDNA HS Assay Kit

Fluorometry Nucleic acid quantification

貨號	數量	價格	交貨時間
A2102	10 assays	–	1 天
12102	100 assays	$105 $67	1 天
13102	100 assays	$125 $79	1 天
52102	500 assays	$290 $147	1 天
53102	500 assays	$389 $176	1 天
72102	1000 assays	$499	1 天

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This kit for DNA quantification is optimized for fluorometers featuring high sensitivity (HS) and a measurements range from 10 pg/uL to 100 ng/uL.The kit includes the concentrated QuDye HS assay reagent, dilution buffer, and DNA standards (0 and 10 ng/uL). The assay is selective towards dsDNA (double-stranded DNA) over RNA and is accurate for initial sample concentrations from 10 pg/uL to 100 ng/uL. The experiment is performed at room temperature and the signal is stable for 3 hours. The assay tolerates contaminants like salts, free nucleotides, detergents, or proteins.

This kit allows to simply dilute the reagent and buffer concentrates and start your measurements. The fluorometer instrument is able to read samples of about 200 uL. For sample preparation mix 180-199 uL of the QuDye HS working solution and 20-1 uL of the DNA sample to be quantified.

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QuDye dsDNA HS Assay Kit manual

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QuDye dsDNA BR Assay Kit

Ready kit for accurate DNA quantification with fluorometer in the broad range from 100 pg/µL to 1000 ng/µL. Common contaminants are well tolerated in the assay, only 1-20 µL of experimental sample is required. Measurements are performed at room temperature.

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參考資料

Bonanno Ferraro, G.; Bonomo, C.; Brandtner, D.; Mancini, P.; Veneri, C.; Briancesco, R.; Coccia, A.M.; Lucentini, L.; Suffredini, E.; Bongiorno, D.; Musso, N.; Stefani, S.; La Rosa, G. Characterisation of microbial communities and quantification of antibiotic resistance genes in Italian wastewater treatment plants using 16S rRNA sequencing and digital PCR. Science of The Total Environment, 2024, 933, 173217. doi: 10.1016/j.scitotenv.2024.173217
Grumov, D.; Kostarnoy, A.; Gancheva, P.; Kondratev, A. A Simple and Rapid Microscale Method for Isolating Bacterial Lipopolysaccharides. International Journal of Molecular Sciences, 2024, 25(12), 6345. doi: 10.3390/ijms25126345
Wu, Y.; Leyk, S.; Torabi, H.; Höhn, K.; Honecker, B.; Tauler, M. D. P. M.; Cadar, D.; Jacobs, T.; Bruchhaus, I.; Metwally, N. G. Plasmodium Falciparum Infection Reshapes the Human MicroRNA Profiles of Red Blood Cells and Their Extracellular Vesicles. iScience, 2023, 26(7), 107119. doi: 10.1016/j.isci.2023.107119
Frolov, A.V.; Akhmetova, L.A.; Vishnevskaya, M.S.; Kiriukhin, B.A.; Montreuil, O.; Lopes, F.; Tarasov, S.I. Amplicon metagenomics of dung beetles (Coleoptera , Scarabaeidae , Scarabaeinae) as a proxy for lemur ( Primates , Lemuroidea ) studies in Madagascar. ZooKeys, 2023, 1181, 29-39. doi: 10.3897/zookeys.1181.107496